FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636380
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT54180.3310966890331097No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36400.2224422200222442No Hit
GTAATAAAAGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG34710.21211454552121145No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31060.1898092130189809No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACT30320.1852870360185287No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29180.17832043901783204No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCTTTTATTA28930.17679267651767927No Hit
GAGTTACAGTGGCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC28210.17239272051723925No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26710.1632261455163226No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA26550.16224837751622484No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG26350.16102616751610263No Hit
ACTTTGCACAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGGAC25650.15674843251567483No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25460.15558733301555872No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT24490.14965961451496596No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24330.14868184651486818No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG23560.14397633801439763No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23140.14140969701414097No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23110.14122636551412265No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23000.1405541500140554No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22520.13762084601376207No Hit
GTGCAAAGTGGATGCATCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTC22460.1372541830137254No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22350.1365819675136582No Hit
TCCCTATCTTTGGTACAGCAAACTACGCGCAGAAGTTCCAGGGCAGAGTC21700.13260978501326098No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT21300.13016536501301654No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21140.12918759701291876No Hit
GACCAGGGAAAGCCCCTAACCTCCTGATCTATGATGCATCCACTTTGCAC21070.128759823512876No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG20930.12790427651279043No Hit
CTTTTATTACTGTGCGAGAGATTGTAGTCCTACCACCTGCTATCGAGACT20810.1271709505127171No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT20550.1255820775125582No Hit
GATCTATGATGCATCCACTTTGCACAGTGGGGTCCCATCAAGGTTCAGTG20000.12222100001222211No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19890.12154878451215488No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19630.119959911511996No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCTGAGCATTAGCAG19380.11843214901184321No Hit
GTCCCAGACCCACTGCCACTGAACCTTGATGGGACCCCACTGTGCAAAGT19190.1172710495117271No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT18900.11549884501154989No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18700.11427663501142767No Hit
TCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTCTCTGCTGAAACCAATT18160.11097666801109768No Hit
CATAACAACCACATCCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT18140.11085444701108545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18080.11048778401104879No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC17870.10920446351092045No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17510.10700448551070045No Hit
CATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT17470.106760043510676No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA17400.10633227001063322No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17130.10468228651046824No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA17120.10462117601046211No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16970.10370451851037046No Hit
ACTTACTACTGTCAACAGAGTTACAGTGGCCGGACGTTCGGCCAAGGGAC16750.10236008751023601No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16750.10236008751023601No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC16450.10052677251005267No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16370.10003788851000378No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)