FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884086
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGACAGACG112080.5948773039022635No Hit
CTATTACTATTCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTC110620.5871281884160277No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC108530.5760352765213478No Hit
GACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCACTA95490.5068239984798996No Hit
TAGTAATAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGAGCGTCC84020.44594567339282815No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG81060.4302351378864871No Hit
GCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAAGACCAC73680.3910649513875693No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64450.3420756801971884No Hit
GTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGACAGACGGCCAC58950.3128838067901359No Hit
TTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCA54820.2909633636681128No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53450.28369193338308335No Hit
GTATCATAATAGATGACCAGTAGAGGGGCCTGGCCTGGCTTCTGCTGGTA52910.28082582217584545No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT52490.278596624570216No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA52040.27620819856418444No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC49470.26256763226307084No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48970.2599138255897024No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA47050.24972320796396766No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT45740.24277023447974244No Hit
GTAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGGTTTACTC44610.2367726313979298No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA43700.23194270325239932No Hit
ATTCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTCC41730.22148670495932776No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA41700.22132747655892565No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCAC40810.21660370068032989No Hit
GCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCAACTCTGGGAACACGG40700.2160198632121888No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT39710.21076532599891934No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG39150.20779306252474675No Hit
GGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGC38890.2064130830545952No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38790.2058823217199215No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA38310.20333466731348782No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36980.19627554156232785No Hit
CCCCTCTACTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCT36680.1946832575583068No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCAGTGTCA35490.18836719767568996No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC35370.18773028407408154No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC34980.18566031486885418No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT34840.184917249000311No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA34450.18284727979508367No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA34020.18056500605598683No Hit
CCCCAGGACAGACGGCCACAATTACCTGTGGGGGGAACAACATTGGGAGT32610.1730812712370879No Hit
GAGTAAACCTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTCTAC32380.17186052016733844No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31600.1677205817568837No Hit
CTACTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCG31300.16612829775286267No Hit
GTAATACATGGCGGTGTCCGAGGTCTTCAGGCTGCTCCACTGCAAGTAGG31090.16501369895004794No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG30090.15970608560331112No Hit
CAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTCCTGATGGTCAGGGT29830.15832610613315953No Hit
TTACTATTCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGAC28870.15323079732029218No Hit
CACTATTACTATTCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCT28680.15222235078441218No Hit
CTATTATTGTCAGGTGTGGAATAGTAATAGTGATCATGTGGTCTTCGGCG28460.15105467584813007No Hit
GTGTGGAATAGTAATAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCT27440.14564091023445852No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG26940.1429871035610901No Hit
ATGTATTACTGTGCGAGATCGACAGACAGCAGCGGCTGGAATGATTCTTT26850.1425094183598838No Hit
GTAATAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGAGCGTCCTA26590.1411294388897322No Hit
TCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGGTTCTCTGGC26370.1399617639534501No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG26260.13937792648530906No Hit
CAATTACCTGTGGGGGGAACAACATTGGGAGTAAACCTGTGCACTGGTAC26170.13890024128410275No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT26130.13868793675023328No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTCTACTGGTCATCTATTATGAT26130.13868793675023328No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG25950.13773256634782063No Hit
GAATAGTAATAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGAGCG25630.13603413007686485No Hit
CCTGAGGGCCGGTCGGTATCATAATAGATGACCAGTAGAGGGGCCTGGCC25470.13518491194138696No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG25360.13460107447324593No Hit
CCTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTCTACTGGTCAT25310.13433569380590907No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC25250.13401723700510484No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG25040.13290263820229012No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24550.13030190766238908No Hit
CCCTCAGTGTCAGTGGCCCCAGGACAGACGGCCACAATTACCTGTGGGGG24530.13019575539545436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24370.12934653725997647No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24230.1286034713914333No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG24180.12833809072409647No Hit
TCCCTGAGCGGTTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACC23690.1257373601841954No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC23340.12387969551283752No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA23070.12244663990921857No Hit
GACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTCCTGATGGTCAGG22870.12138511723987122No Hit
ATTATTGTCAGGTGTGGAATAGTAATAGTGATCATGTGGTCTTCGGCGGA22860.12133204110640385No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG22660.1202705184370565No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT22560.1197397571023828No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21640.11485675282338491No Hit
CTCTACTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAG21400.11358292562016808No Hit
ATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCC21170.11236217455041861No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG20840.11061066214599546No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA20760.11018605307825652No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCA20420.108381464540366No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT20320.10785070320569232No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20230.10737301800448601No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA19790.10503766813192178No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTC19540.10371076479523758No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC19280.10233078532508602No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAACCGCTCAGGGATCCCT19240.10211848079121653No Hit
GACCAGTAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGGTT18910.10036696838679339No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33200.080.072881
ATCAACG41200.064.49053
TCAACGC41050.064.375334
TATCAAC42950.061.527532
CAACGCA44100.059.923075
AACGCAG46000.057.4434136
CGCAGAG51800.051.010178
ACGCAGA55450.047.5225837
GCAGAGT59650.044.2971849
GTACATG58650.041.51891
CATGGGA29900.040.6988454
ACATGGG59250.040.2259453
ATGGGAG26150.039.3759425
CAGCGCT6950.037.293939
TACATGG71000.034.3801652
GTACACG10000.033.12265
TATACGT900.00375267832.0025145
TACACGG10400.031.846126
TATTGAG28800.031.5024745
TTATTGA31250.029.4935154
Produced by FastQC (version 0.11.5)