FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884086
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTACTATTCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTC106530.5654200498278741No Hit
CCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGACAGACG99690.5291159745361942No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC95590.5073547598145732No Hit
GACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAAGACCACATGATCACTA86520.4592147067596702No Hit
TAGTAATAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGAGCGTCC76540.4062447255592367No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG72610.38538580510656095No Hit
GCCTTGGGCTGACCTAGGACGCTCAGCTTGGTCCCTCCGCCGAAGACCAC66450.3526909068906621No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57210.3036485595668138No Hit
GTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGACAGACGGCCAC53740.285231141253637No Hit
GTATCATAATAGATGACCAGTAGAGGGGCCTGGCCTGGCTTCTGCTGGTA51200.2717498033529255No Hit
TTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCA50650.26883061601222025No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA49730.2639476117332224No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49150.260869195992115No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT48810.2590646074542245No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC45380.24085949367491719No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44540.23640109846365825No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA42890.22764353644154248No Hit
GTAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGGTTTACTC42540.2257858717701846No Hit
ATTCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTCC40260.21368451333962463No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40250.21363143720615724No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA40130.21299452360454885No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA37080.19680630289700152No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA36500.19372788715589415No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT36030.19123330888292786No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCAC35600.18895103514383102No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT35440.18810181700835313No Hit
GCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCAACTCTGGGAACACGG34910.18528878193458262No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34640.18385572633096367No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC34270.18189190939267103No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG34150.1812549957910626No Hit
GGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGC33670.17870734138462893No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT32100.17037438843025213No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA31930.16947209416130685No Hit
CCCCTCTACTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCT31930.16947209416130685No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCAGTGTCA31310.16618137388633003No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA30920.16411140468110266No Hit
GTAATACATGGCGGTGTCCGAGGTCTTCAGGCTGCTCCACTGCAAGTAGG30530.16204143547587532No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC30240.16050222760532162No Hit
CCCCAGGACAGACGGCCACAATTACCTGTGGGGGGAACAACATTGGGAGT30080.15965300946984373No Hit
GAGTAAACCTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTCTAC29770.1580076493323553No Hit
TTACTATTCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGAC28610.1518508178501406No Hit
CTACTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCG28340.15041776224652165No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA28260.1499931531787827No Hit
CAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTCCTGATGGTCAGGGT28150.14940931571064164No Hit
GTGTGGAATAGTAATAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCT27420.14553475796752377No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG26680.1416071240909385No Hit
CTATTATTGTCAGGTGTGGAATAGTAATAGTGATCATGTGGTCTTCGGCG26490.14059867755505853No Hit
CACTATTACTATTCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCT25830.13709565274621222No Hit
ATGTATTACTGTGCGAGATCGACAGACAGCAGCGGCTGGAATGATTCTTT25210.13380493247123537No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG25130.13338032340349645No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT24820.13173496326600803No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC24630.13072651673012803No Hit
CCTGAGGGCCGGTCGGTATCATAATAGATGACCAGTAGAGGGGCCTGGCC24400.12950576566037855No Hit
GTAATAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGAGCGTCCTA24380.1293996133934438No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG23970.12722349192128174No Hit
CAATTACCTGTGGGGGGAACAACATTGGGAGTAAACCTGTGCACTGGTAC23950.127117339654347No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG23940.12706426352087963No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGG23930.12701118738741227No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG23030.1222343353753491No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.122075106974947No Hit
GAATAGTAATAGTGATCATGTGGTCTTCGGCGGAGGGACCAAGCTGAGCG22650.1202174423035891No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22600.11995206163625229No Hit
TCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGGTTCTCTGGC22530.11958052870198069No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22450.11915591963424174No Hit
CCTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTCTACTGGTCAT22240.118041320831427No Hit
TCCCTGAGCGGTTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACC22000.11676749362821018No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG21730.11533443802459124No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTCTACTGGTCATCTATTATGAT21590.11459137215604807No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA21320.11315831655242914No Hit
ATTATTGTCAGGTGTGGAATAGTAATAGTGATCATGTGGTCTTCGGCGGA21050.11172526094881019No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC20970.11130065188107124No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT20900.11092911894679967No Hit
CCCTCAGTGTCAGTGGCCCCAGGACAGACGGCCACAATTACCTGTGGGGG20850.11066373827946283No Hit
GACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTCCTGATGGTCAGG20750.11013297694478916No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG20410.10832838840689862No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG19990.10609919080126916No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19610.10408229772950917No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCA19100.10137541492267338No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18990.10079157745453234No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18970.1006854251875976No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)