FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2795470
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT77600.2775919612802141No Hit
TCTATGATGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGC57250.20479561576407546No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53530.19148837225940538No Hit
CTCTCAGACTGTCCATTTGCAGATAGAGGGTGTTCTCGGAATTGTCTCTG51530.1843339402676473No Hit
GTCTGAGAGAACATGACACGGCCTTTTATTATTGTGTGAAGTCTAAGGGT50700.1813648509910677No Hit
GTATATTGCTGACAGTAGTAAACTGCAGAATCTTCAGACTGGAGGCTGCT47270.16909500012520257No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC46890.16773565804676852No Hit
GCAATATACTCACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTTG45570.16301373293220817No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAGAGAGTCACCCTCTCCT44390.1587926180570709No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43900.15703978221909018No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37390.13375210608591756No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC35870.12831473777218141No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA35700.127706611052882No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAAGTGGTGGAGTCTGGGGGAGGCG35400.12663344625411826No Hit
CTCCTGCAGGGCCAGTCAGAGTATTAGAATCAACTTAGCCTGGTACCAGC34210.12237655921902221No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34160.12219769841922824No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33410.11951478642231897No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC33080.11833430514367888No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33060.11826276082376129No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31680.11332620274944821No Hit
TTACTACTGTCAGCAATATACTCACTGGCCTCCGTACACTTTTGGCCAGG31300.11196686067101419No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31270.11185954419113782No Hit
GTCTAAGGGTTTGGAGTGGTTCACAATCGTCGGCTACTACTTTGACTACT31030.11100101235212684No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA30950.11071483507245651No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT30810.11021402483303346No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30620.10953435379381642No Hit
GTATTAGAATCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGTCTCCC30620.10953435379381642No Hit
GAGTATTAGAATCAACTTAGCCTGGTACCAGCAAAAACCTGGCCAGTCTC29290.10477665651929728No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.10445470707966817No Hit
CCCTTAGACTTCACACAATAATAAAAGGCCGTGTCATGTTCTCTCAGACT29140.10424007411991543No Hit
ATATTACGCAGACTCCGTCAAGGGCCGATTCACCATCTCCAGAGACAATT29030.10384658036036874No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG28080.10044822516428364No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTTTGGGACAGAATTCACT28070.10041245300432486No Hit
GCGTAATATGTATTACTTCCGTCATGCGAAATAACTGCCAGCCACTCCAG28060.10037668084436607No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)