FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755737
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27510.3640155239190353No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCT21040.2784037303982735No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18920.25035164349502537No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17390.2301065053054171No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA13830.18300017069430238No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCG13660.18075071089545702No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12930.17109126587688572No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12920.1709589447122478No Hit
CTACAAACTCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAA12590.166592346279195No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12310.16288735366933205No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCCGTACACTTTTGGCCAGG11990.15865307640091725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11680.1545511202971404No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11250.14886131021770801No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11050.14621488692494877No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10900.14423006945537933No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT10800.1429068578089997No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA10270.13589383608318767No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG9770.12927777785128955No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9300.12305868311330528No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8850.11710423070459698No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG8740.11564869789357937No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT8310.10995888781414698No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC8180.10823871267385347No Hit
GCATAGTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC7930.1049306835579044No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7900.10453372006399052No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA7900.10453372006399052No Hit
GTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCG7730.10228426026514514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16650.076.7355351
TCAACGC18900.066.659624
ATCAACG18900.066.278713
CAACGCA19150.065.413455
GGTATCA6500.064.409831
AACGCAG20400.061.7581756
TATCAAC20500.061.150242
ACGCAGA23250.053.8781787
CGCAGAG23850.052.5227478
CATTCGT900.003753434431.9987379
GCAGAGT40950.031.8229169
GTATAGA2151.8879291E-830.216321
CAGAGTA26450.026.4034929
ACATTAG5850.025.8434227
GTACATG21450.024.5659241
TACGAGT2351.4136203E-624.5080496
TATACTG3251.6098056E-924.3666575
TATACAG3551.6916601E-1024.3354555
CTCCAAC7150.024.1828782
TGGATAC20250.023.8197045
Produced by FastQC (version 0.11.5)