FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035637
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATTACTGTGCGAGACATGATCATTCGGACCACGGTGCCCCCAACTG33890.32723821184449764No Hit
GTCTCTGGTGCCTCCATCAGCAGTGCTAAACTCTGCTGGGGCTGGATCCG32380.31265781350029015No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT31150.3007810651801741No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30560.2950840883436957No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT29950.28919399364835363No Hit
GTAATAAATCGCCGTGTCTGCGGCGGTCACGGAGTTCAGTGTCAGGGAGA29520.2850419596827846No Hit
TCTCTAGGGTGTCTAACTTACAAAGTGGGGTCCCATCAAGGTTCAGCGGC28080.2711374738446No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA24880.24023861642641195No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT23200.22401671628186323No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC22650.2187059751631122No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22020.2126227626089064No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT21410.20673266791356432No Hit
TCCGTAGACACGTCCAGGAATCAGTTCTCCCTGACACTGAACTCCGTGAC20830.20113225000651774No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG20530.1982354821235626No Hit
ATTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA20070.19379377136969805No Hit
GTCTTACGGTGGGATCCCTGACTACAACCCGTCCCTCGAGAGTCGAGTCC19260.18597249808571922No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18870.18220669983787754No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGCAGTGCTAAACTCTGCTGGG17750.17139209974151173No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC17270.16675727112878355No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG16130.15574955317355405No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT15510.14976289954878014No Hit
TCCATCAGCAGTGCTAAACTCTGCTGGGGCTGGATCCGCCAGCCCCCGGG15490.14956978168991644No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA15340.14812139774843888No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15260.14734892631298419No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG15140.14619021915980213No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14980.14464527628889273No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT14980.14464527628889273No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG14960.14445215843002904No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA14880.14367968699457434No Hit
GTGCTAAACTCTGCTGGGGCTGGATCCGCCAGCCCCCGGGGAAGGGCCTG14310.1381758280169596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.1380792690875278No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG14160.13672744407548204No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13860.13383067619252692No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13820.13344444047479956No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13690.1321891743921857No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGTCACGGTCACCACGCTGCT13570.13103046723900363No Hit
ATGCACCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTT13110.1265887564851391No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA13010.12562316719082073No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA12950.1250438136142297No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA12920.12475413682593418No Hit
GAATATTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC12910.12465757789650235No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12900.1245610189670705No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12890.12446446003763867No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAAC12630.12195392787241088No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12610.12176081001354722No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12550.1211814564369562No Hit
GTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGATCTCATGTG12350.11925027784831943No Hit
CCCTAGAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG12330.11905715998945578No Hit
TTATTACTGTGCGAGACATGATCATTCGGACCACGGTGCCCCCAACTGGT12180.11760877604797819No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12100.1168363046125235No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG11910.11500168495331858No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC11810.1140360956590002No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACGACGGTGAGGACGC11530.11133244563490877No Hit
GTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11500.11104276884661324No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11280.10891847239911281No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11280.10891847239911281No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11230.10843567775195362No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGA11180.10795288310479444No Hit
GAGATAGAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGC11100.10718041166933974No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCGTGCACCAGG11090.1070838527399079No Hit
GTGTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACG10860.10486299736297564No Hit
GTGCGAGACATGATCATTCGGACCACGGTGCCCCCAACTGGTTCGACCCC10770.1039939669980891No Hit
CTGTTGAACTGCTCCTCCCGTGGCTTTGTCTTGGCATTATGCACCTCCAT10720.10351117235092991No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATAC10710.10341461342149808No Hit
GGCCTGGAGTGGATTGCAAGCATGTCTTACGGTGGGATCCCTGACTACAA10520.10157999376229315No Hit
GACTACAACCCGTCCCTCGAGAGTCGAGTCCTCATATCCGTAGACACGTC10480.1011937580445658No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10410.10051784553854294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)