FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences830525
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGACCAGGGAGG33420.4023960747719816No Hit
GATTATTACTGTGCAGCATGGGATGACGGCCTGAGTGGTCCGGGGTTCGG24740.29788386863730776No Hit
GCCTTGGGCTGACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACCCCGG18050.21733241022244965No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16080.1936124740375064No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15480.18638812799133078No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCTGGGACCC13660.16447427831793143No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13580.16351103217844135No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13570.1633906264110051No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCACCCTCAGCGTCT13340.1606212937599711No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13310.16026007645766233No Hit
GTAGTACGTGGCCGTGTCTGCAGCGGTCACGGAGTTCAACTTCAGGGAGA12530.15086842659763403No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12420.14954396315583518No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC11630.1400319075283706No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10900.1312422865055236No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10730.1291953884591072No Hit
GCATGGGATGACGGCCTGAGTGGTCCGGGGTTCGGCGGAGGGACCAAGTT10720.12907498269167092No Hit
GTGTTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCTC10520.12666686734294572No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10470.12606483850576441No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAC10250.1234159116221667No Hit
GTATACTGGTACCAGCAGGTCCCTGGAACGGCCCCCAAACTCCTCATCTT10220.12305469431985792No Hit
CTTCATGCGTGACCTGGCAGGTGTAGCTTCTGTGGGACTTCCACTGCTCA10040.12088739050600524No Hit
GTCTCTGGTGGCTCCGTCAGTAGTGGTAGTTACTATTGGAGCTGGATGCG9910.11932211552933385No Hit
GACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACCCCGGACCACTCAGG9900.11920170976189759No Hit
ACCCAGGACGGTCAACTTGGTCCCTCCGCCGAACCCCGGACCACTCAGGC9630.11595075404111858No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9570.115228319436501No Hit
GGTACCAGCAGGTCCCTGGAACGGCCCCCAAACTCCTCATCTTTAGGAGT9560.11510791366906474No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9460.11390385599470215No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGGTGTAGCTTCTGTGGGACTTCCA9370.11282020408777581No Hit
GTCCGGGGTTCGGCGGAGGGACCAAGTTGACCGTCCTGGGTCAGCCCAAG9350.11257939255290329No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9310.11209776948315825No Hit
ACCCCGGACCACTCAGGCCGTCATCCCATGCTGCACAGTAATAATCAGCC9090.10944884259956052No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9030.10872640799494296No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGTAT8960.10788356762288914No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8910.10728153878570784No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT8890.10704072725083531No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8880.10692032148339906No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8570.10318774269287498No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8440.10162246771620362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.071.739671
GTATCAA19650.062.0806351
ATCAACG21350.056.31173
TCAACGC21550.056.123154
CAACGCA22050.054.5240255
TATCAAC22450.053.5944982
AACGCAG23250.052.019526
ACGCAGA25800.046.878067
CGCAGAG26450.045.4538658
GCAGAGT29650.040.548229
CTTACGC850.002838010733.8782583
ATATACG4100.029.9293351
ATACGAG4200.029.1393363
TACATGG21650.028.2866212
GTACATG22250.027.5753421
TAGTACG7850.027.534152
AATCCGC1050.00797712427.4252576
TACGAGT4500.027.1967144
TATTGAG8550.026.944115
CACCGGT1358.4208255E-426.6634467
Produced by FastQC (version 0.11.5)