FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1154982
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTACCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAACTCA69730.6037323525388274No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT55910.48407680812341664No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35950.3112602620646902No Hit
GTAATAAACAGCCGTGTCCTCGTCTCTCAGGCTGTCCATTTGCAAATACA33910.2935976491408524No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26340.22805550216367007No Hit
GTTTATTACTGTGCGAGAGGGCAAGACTACGGTGACTACTGTGACTGGGG25420.22009001006076284No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC24110.20874784195771015No Hit
CAGTATAATAGTTACCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAACT23650.20476509590625652No Hit
CCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAACTCAAACGAACTGTGG20360.17627980349477307No Hit
GATCTATGCTGCCTCCAGTTTGCAAGGTGGGGTCCCATCAAAGTTCAGCG19550.16926670718677866No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19190.1661497754943367No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA18800.16277309949419125No Hit
GTAGGAGACAGAGTCACCATTACTTGTCGGGCGAGTCAGGGCATCAGCAA18130.15697214328881318No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT17990.15576000318619684No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17950.15541367744259219No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17720.15342230441686536No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT15670.13567311005712643No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15170.13134403826206817No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC14520.1257162449284924No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13810.11956896297950963No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13600.11775075282558516No Hit
CACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAAC13550.11731784564607935No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13250.11472040256904437No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13020.11272902954331755No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG12930.11194979662020707No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12870.11143030800480007No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGG12610.10917919067136977No Hit
GATTTCACACTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA12500.10822679487645695No Hit
ATCTTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG12490.10814021344055579No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATTACTTGTCGGGCGAGTCAGG12420.10753414338924763No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12310.10658174759433481No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12080.104590374568608No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAGTCACCATTACTT11990.10381114164549751No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT11700.10130028000436371No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11670.10104053569666022No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG5550.060.9621439
GGTATCA6900.057.4283451
ATCAACG14800.057.3952063
TCAACGC15100.056.731644
GTGCTAG7200.053.034211
CAACGCA16350.052.834655
AACGCAG16850.051.2668536
TATCAAC17400.049.646352
GTATCAA18250.048.9521181
CGCAGAG18850.045.063618
CTAGAGA8950.041.824974
GTACATG29250.037.932161
GCTAGAG9300.037.928743
TACATGG29150.037.043162
AGAATAC9200.036.7760738
GCAGAGT23600.035.6885729
CTATTAT29700.035.1742021
TGCTAGA11200.034.708122
TATACTG30950.033.9584245
AATAGTT34500.033.5940325
Produced by FastQC (version 0.11.5)