FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164887
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCTTTTATTA33460.28723816129804863No Hit
GTAATAAAAGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGA33330.2861221732236689No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA29320.25169823339087827No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24150.20731624612516064No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT23840.2046550437939474No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23700.2034532104830769No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22270.1911773416648997No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22130.18997550835402918No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21700.18628416318492697No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20800.17855809190075947No Hit
CTTTTATTACTGTGCGAGAGAGGGGGATAATAGTGGTTATTCCTTTGACT19580.168084973048888No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19350.16611053260960076No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT19060.16362102075136903No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG18890.16216165173102629No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18190.1561524851766738No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG17160.14731042581812656No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC17140.14713873534514507No Hit
CTATTATCCCCCTCTCTCGCACAGTAATAAAAGGCCGTGTCCTCAGATCT16730.14361908064902432No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG16090.13812498551361635No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG16050.13778160456765334No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15840.1359788546013476No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT15710.13486286652696786No Hit
CCATGTAGACTGTGTTCGTGGATTCGTCCGCGGTGATCGTGACTCTGTCC15700.13477702129047708No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACC15240.1308281404119026No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACCACCA14970.1285103190266524No Hit
CATGTAGACTGTGTTCGTGGATTCGTCCGCGGTGATCGTGACTCTGTCCC14780.12687925953332813No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14520.12464728338456862No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14370.1233596048372074No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14030.12044086679652188No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13900.11932487872214216No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA13860.11898149777617914No Hit
ACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCTTTTATTACTGT13480.11571937878953066No Hit
TTTTATTACTGTGCGAGAGAGGGGGATAATAGTGGTTATTCCTTTGACTC13370.1147750811881324No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG13330.1144317002421694No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGT13140.11280064074884516No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12990.11151296220148392No Hit
GTGCGAGAGAGGGGGATAATAGTGGTTATTCCTTTGACTCTTGGGGCCAG12950.11116958125552093No Hit
CTACTATGGTGGTGGTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC12890.11065450983657643No Hit
TTATTACTGTGCGAGAGAGGGGGATAATAGTGGTTATTCCTTTGACTCTT12790.10979605747166891No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12700.10902345034325218No Hit
GGATAATAGTGGTTATTCCTTTGACTCTTGGGGCCAGGGAACCCTGGTCA12680.10885175987027068No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12600.10816499797834467No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12200.10473118851871469No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG12120.10404442662678869No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12060.10352935520784419No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11960.10267090284293669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11920.1023275218969737No Hit
TCCCTGTCTTTGGTAGAGGAAACTACGCACAGAACTTCCGGGACAGAGTC11860.1018124504780292No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11850.10172660524153845No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11790.10121153382259396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7950.078.894981
GTATCAA20350.076.522051
ATCAACG25000.062.2728963
TCAACGC25650.060.6948244
TATCAAC26300.058.920712
CAACGCA26550.058.0944375
AACGCAG27800.055.422736
CGCAGAG32550.047.3267948
CAGCGCT7600.042.6229259
GCAGAGT37000.041.6347929
ACGCAGA37300.041.2999237
GAATACG2550.036.6984339
TATTGAG9250.034.2843745
TACACCG1352.2303557E-532.033385
GCAGCGC9850.031.425098
GGCAGCG10750.030.1334157
GTGCTAG3600.030.039041
GTACATG30450.029.358351
ACATGGG30700.028.6422253
CAGAGTA36100.028.315589
Produced by FastQC (version 0.11.5)