FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164887
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCTTTTATTA33080.28397604231140017No Hit
GTAATAAAAGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGA31550.27084172112831545No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA27160.2331556623088763No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24750.2124669603146056No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23200.1991609486585394No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT22490.1930659368676962No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22100.18971797264455695No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21160.18164852041442647No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20720.17787133000883348No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG19940.17117540156255498No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19380.16636806831907303No Hit
CTTTTATTACTGTGCGAGAGAGGGGGATAATAGTGGTTATTCCTTTGACT18480.15864199703490553No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT18130.1556374137577293No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC17890.15357712808195131No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC16260.1395843545339591No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16150.13864005693256085No Hit
CTATTATCCCCCTCTCTCGCACAGTAATAAAAGGCCGTGTCCTCAGATCT16100.1382108307501071No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG15750.13520624747293086No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15530.13331765227013434No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15350.13177243801330085No Hit
CCATGTAGACTGTGTTCGTGGATTCGTCCGCGGTGATCGTGACTCTGTCC14820.12722264047929113No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14260.12241530723580915No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA14040.12052671203301264No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT14030.12044086679652188No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACC13860.11898149777617914No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13750.11803720017478089No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13710.11769381922881791No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG13590.11666367639092891No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACCACCA13590.11666367639092891No Hit
CATGTAGACTGTGTTCGTGGATTCGTCCGCGGTGATCGTGACTCTGTCCC13560.11640614068145665No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG13450.11546184308005841No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13330.1144317002421694No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13060.11211387885691917No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12710.10910929557974294No Hit
CTACTATGGTGGTGGTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCC12210.10481703375520544No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12180.10455949804573318No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11990.10292843855240895No Hit
GGATAATAGTGGTTATTCCTTTGACTCTTGGGGCCAGGGAACCCTGGTCA11950.10258505760644594No Hit
GTGCGAGAGAGGGGGATAATAGTGGTTATTCCTTTGACTCTTGGGGCCAG11890.10206998618750145No Hit
TTTTATTACTGTGCGAGAGAGGGGGATAATAGTGGTTATTCCTTTGACTC11860.1018124504780292No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG11840.1016407600050477No Hit
TTATTACTGTGCGAGAGAGGGGGATAATAGTGGTTATTCCTTTGACTCTT11830.10155491476855694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15750.068.518561
ATCAACG16350.062.4623953
TCAACGC16300.062.212774
GGTATCA6050.058.2690851
CAACGCA17750.057.1305965
TATCAAC18450.056.1397132
AACGCAG19150.052.9152346
CGCAGAG21400.047.4476138
GCAGAGT25400.040.2590649
ACGCAGA27800.036.2653857
TATTGAG10550.034.0851755
GGTTTAT8900.033.1429671
TTATTGA11650.032.718844
GTACATG30050.031.6029051
CAGCGCT8000.031.5055529
ATTGAGT9450.031.1804566
ACATGGG30650.030.5042883
GGCAGCG8750.027.9820737
TTTATTG15300.027.7337473
GTACACG13350.027.4749495
Produced by FastQC (version 0.11.5)