FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413549
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC46670.3301618833163902No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39130.27682096623463354No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTG35150.24866488533471426No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG32740.23161560016667268No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG29700.2101094479215082No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28380.20077125023610787No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAAAGAAGTT27210.19249421137859388No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA26700.1888862713637801No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG26630.18839106391076646No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26360.1864809780205709No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT24930.17636459719472053No Hit
GAAATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA23440.16582375283771558No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23420.16568226499399738No Hit
GATTATTACTGCAGCTCATCTACAAACAGGAACACTTGGGTTTTCGGCGG23230.1643381304786746No Hit
CTCCTGCACTGGAACCAGCAAAGAAGTTGGTGGTTATGACTATGTCTCCT22710.16065944654200173No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22240.15733448221462432No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG22060.15606109162116064No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22030.15584885985558336No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT21970.1554243963244288No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC21620.15294835905936052No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21490.1520286880751923No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTGT21190.14990637041941948No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC20380.1441761127488329No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT20220.1430442099990874No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT19770.1398607335154282No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC19410.13731395232850083No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA19230.13604056173503712No Hit
GATTAAGACTGATGGGAGTGTAACAAGCTACGCGGACTCCGTGAAGGGCC19150.13547461036016437No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTG19040.13469642721971434No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18660.13200815818906877No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18550.13122997504861875No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA18230.12896616954912776No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17610.12458004639386396No Hit
GTATATTTCTGTGCACGAGATCTCAGGGGATCCAACTACTGGGGCCAGGG17560.12422632678456848No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16800.11884978872327738No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16660.11785937381725005No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG16530.11693970283308183No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16460.11644449538006818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.1138269702712817No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT16020.11333176281826805No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15890.11241209183409985No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA15840.11205837222480437No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT15760.11149242084993163No Hit
ATATACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACAGCG15640.11064349378762249No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15240.10781373691325877No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15150.1071770416165269No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC15080.10668183416351325No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG14790.10463026042959954No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14500.10257868669568582No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT14390.10180050355523579No Hit
GGTTATGACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGCCCCCAA14300.10116380825850395No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC14290.10109306433664486No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)