FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973850
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCACCGGCTGTTCCCCGCGAATTTGGCAACTGGTTCGACCCCTGG32210.33074908866868613No Hit
GTGCTATACGGCTCAGTAAGTTAACTCTCGCACAATAATATACGGCCGTG29280.3006623196590851No Hit
ATATTATACTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA27150.2787903681265082No Hit
ATAATATACGGCCGTGTCTGCGGCGGTCACAGAGCCCAGCTTCAGGGAGA27070.2779688863788058No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTACTACTGGGGCTGGATCCGGCA17540.1801098731837552No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16160.1659393130358885No Hit
GTATATTATTGTGCGAGAGTTAACTTACTGAGCCGTATAGCACCGGCTGT15810.1623453303896904No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15450.15864866252502952No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15300.15710838424808748No Hit
ATATACGGCCGTGTCTGCGGCGGTCACAGAGCCCAGCTTCAGGGAGAACT15270.15680032859269907No Hit
GTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCT15010.15413051291266622No Hit
ATTCTATACAGTTCCCATAACAAGAACTTCTTAGCTTGGTACCAGCAGAA14400.14786671458643527No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14390.14776402936797248No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12860.13205319094316376No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12620.12958874570005646No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12490.12825383786004005No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12290.126200133490784No Hit
ATATTATTGTGCGAGAGTTAACTTACTGAGCCGTATAGCACCGGCTGTTC11990.12311957693689993No Hit
GGTTACTACTGGGGCTGGATCCGGCAGCCCCCTGGGAAGGGGCTGGAGTG11760.12075781691225548No Hit
ATCATAGTGGGACCACCTCCTACCACCCGTCCCTCCAGAGTCGAGTCACC11100.11398059249371052No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11080.11377522205678492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.11346716640139652No Hit
CAATAATATACGGCCGTGTCTGCGGCGGTCACAGAGCCCAGCTTCAGGGA11020.11315911074600811No Hit
GCCGTATATTATTGTGCGAGAGTTAACTTACTGAGCCGTATAGCACCGGC10960.1125429994352313No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10910.1120295733429173No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10610.10894901678903321No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAACC300.001979950872.088323
GGTATCA7250.069.6060941
GTATCAA18500.064.687981
TCAACGC19850.058.469624
ATCAACG20150.057.5991063
CAACGCA21050.054.7939725
AACGCAG21900.053.3173876
AATACGG701.7556718E-551.4837156
CGCAGAG25650.045.7540638
GCAGAGT29950.038.942639
ACGCAGA30750.037.9450767
TATCAAC31800.037.1776242
GTACATG24000.032.7417951
ACATGGG24150.032.2382553
TACATGG25100.031.0180842
GTACACG8100.030.2592955
TATACTA14200.029.9521885
CATGGGG17800.028.3493394
CTATACG13500.028.3013424
GCTATAC14550.028.2407863
Produced by FastQC (version 0.11.5)