FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973850
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGCACCGGCTGTTCCCCGCGAATTTGGCAACTGGTTCGACCCCTGG31310.32150741900703395No Hit
GTGCTATACGGCTCAGTAAGTTAACTCTCGCACAATAATATACGGCCGTG28950.29727370744981263No Hit
ATATTATACTACTCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA27500.28238435077270624No Hit
ATAATATACGGCCGTGTCTGCGGCGGTCACAGAGCCCAGCTTCAGGGAGA23910.24552035734456026No Hit
GTATAATATTGCTGACAGTAATAAATTGCCACATCTTCAGCCTGCAGGCT19320.19838784207013402No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTACTACTGGGGCTGGATCCGGCA17800.18277968886378806No Hit
GTATATTATTGTGCGAGAGTTAACTTACTGAGCCGTATAGCACCGGCTGT16870.17322996354674744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16210.1664527391282025No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15690.16111310776813678No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14830.15228217898033578No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14540.1493043076449145No Hit
ATATACGGCCGTGTCTGCGGCGGTCACAGAGCCCAGCTTCAGGGAGAACT14330.14714791805719565No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12880.13225856138008935No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12350.1268162448015608No Hit
ATATTATTGTGCGAGAGTTAACTTACTGAGCCGTATAGCACCGGCTGTTC12100.12424911433999075No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11940.12260615084458593No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11920.12240078040766031No Hit
GGTTACTACTGGGGCTGGATCCGGCAGCCCCCTGGGAAGGGGCTGGAGTG11740.12055244647532987No Hit
ATTCTATACAGTTCCCATAACAAGAACTTCTTAGCTTGGTACCAGCAGAA11520.11829337166914823No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10840.11131077681367768No Hit
GCCGTATATTATTGTGCGAGAGTTAACTTACTGAGCCGTATAGCACCGGC10640.10925707244442162No Hit
ATCATAGTGGGACCACCTCCTACCACCCGTCCCTCCAGAGTCGAGTCACC10410.10689531241977718No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10230.10504697848744673No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10220.10494429326898393No Hit
CAATAATATACGGCCGTGTCTGCGGCGGTCACAGAGCCCAGCTTCAGGGA10050.10319864455511629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12050.059.2447364
GTATCAA12500.058.3047371
ATCAACG12500.056.5350343
CAACGCA14350.049.754195
AACGCAG14350.049.6978726
GGTATCA6450.045.868721
CGCAGAG16650.042.8371058
GCAGAGT20000.036.0239689
TACGGCT12800.035.4592677
TATACTA14250.035.4266055
CTATACG13050.035.364794
ATTATAC15200.035.1066783
GCTATAC14500.032.8229453
TTATACT15200.032.7346044
ACGCAGA21800.032.717337
TATACGG27000.032.0526435
GTGCTAT14900.031.9631961
ATACGGC27600.031.842366
CGGCTCA14550.031.6911899
TATCAAC23050.031.6007082
Produced by FastQC (version 0.11.5)