FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686510
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGTACTGGGCGCCCTATTCCTTTCGCACAGAAATATACGGCCGTGT29490.4295640267439659No Hit
GTATAATCTTTACTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC25030.3645977480298903No Hit
GTACTGGGAACTACCCGGGCCTACTGGTACTTCGATCTCTGGGGCCGTGG24060.35046831073108914No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23280.3391064951712284No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20030.29176559700514193No Hit
AGATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCT16680.24296805581856054No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC16310.23757847664272916No Hit
GAAATATACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA14250.20757163042053284No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13330.19417051463197912No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGC13220.19256820730943466No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13040.18994624987254374No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12480.18178904895777193No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11530.16795094026306973No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11240.16372667550363432No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG10490.15280185284992207No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10310.15017989541303114No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT10240.14916024529868466No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATT10030.14610129495564522No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10010.14580996635154622No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9990.14551863774744722No Hit
GTATATTTCTGTGCGAAAGGAATAGGGCGCCCAGTACTGGGAACTACCCG9980.14537297344539774No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG9950.14493598053924925No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9890.14406199472695225No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9740.1418770301962098No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGACTTTCAGGTGTT9610.13998339426956635No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9590.13969206566546735No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9460.13779842973882392No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9310.13561346520808146No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA9160.133428500677339No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8940.13022388603225007No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC8890.1294955645220026No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCAGCTATGCCATGAACTGGG8800.12818458580355713No Hit
GATTATACTGTTGGCAGTAATAAGTTGCAGAATCATCAGGCTGCAGGCTG8770.12774759289740864No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8770.12774759289740864No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG8750.12745626429330964No Hit
ACTTATTACTGCCAACAGTATAATCTTTACTGGACGTTCGGCCAAGGGAC8620.12556262836666618No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8610.1254169640646167No Hit
ATATACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG8420.12264934232567624No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8400.12235801372157726No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTA8350.12162969221132978No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8190.11929906337853782No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGCAGCTATGCC8180.11915339907648831No Hit
GTACTGGGCGCCCTATTCCTTTCGCACAGAAATATACGGCCGTGTCTTCG8150.11871640617033984No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8100.11798808466009236No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8080.11769675605599336No Hit
TTACTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGG8030.11696843454574588No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7970.11609444873344889No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC7960.1159487844313994No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA7940.1156574558273004No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG7820.11390948420270644No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG7780.11332682699450845No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGTTGTCGGAGTCTGGGGGAGGCG7760.11303549839040945No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7740.11274416978631047No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA7660.11157885536991448No Hit
GCGTAGTATGTTCTACTACCAGTACCACTAATAGCTGAGACCCACTCCAG7560.11012221234941953No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7540.10983088374532055No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7530.10968521944327103No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT7530.10968521944327103No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA7480.10895689793302356No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7370.1073545906104791No Hit
GTGTGAGGTGCAGTTGTCGGAGTCTGGGGGAGGCGTGGTACCGCCTGGGG7340.1069175977043306No Hit
GTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA7280.10604361189203361No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA7210.10502396177768715No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7160.10429564026743965No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7150.10414997596539016No Hit
CCCTATTCCTTTCGCACAGAAATATACGGCCGTGTCTTCGGCTCTCAGGC7120.10371298305924168No Hit
GCCGTATATTTCTGTGCGAAAGGAATAGGGCGCCCAGTACTGGGAACTAC7120.10371298305924168No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG7090.10327599015309318No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7080.10313032585104369No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC6920.10079969701825174No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA6900.10050836841415274No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG6890.10036270411210325No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)