FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733458
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17230.2349146099708504No Hit
GTAATAAACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG16260.2216895854977381No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12870.1754701700710879No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12650.17247067998440263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.16892582806377462No Hit
GTTTATTACTGTGCGAGGAAATATTGTACTACTTCCTATTGCCCAGGTCA12380.16878948760528892No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11910.16238148605646133No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11690.15938199596977606No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGTA11680.15924565551129036No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11160.15215595167003426No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11080.15106522800214872No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10740.14642965241363515No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10340.1409760340742074No Hit
ACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTTTATTACTGT10330.14083969361572168No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10060.13715850123660794No Hit
GTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGT9810.13374998977446562No Hit
CCCCTGGACAGGGGCCTGAGTGGATGGGAGGGATCGTCCCTTTCTTTGAT9470.12911441418595201No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG9180.12516054088986692No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9020.1229790935540958No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8360.11398062329404002No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG7880.10743628128672672No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG7810.10648189807732686No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7800.10634555761884117No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7610.10375508890761297No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT7610.10375508890761297No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7560.10307338661518452No Hit
GCCTACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTTTATTA7500.10225534386427033No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7400.10089193927941341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16850.065.894314
ATCAACG17400.063.8114473
CAACGCA17650.062.9075975
AACGCAG18500.060.3328136
GTATCAA21800.052.948981
CGCAGAG22350.049.5940368
ACGCAGA24200.046.100187
TATCAAC29050.039.461912
TAATACG800.002092503536.049324
GTACATG20200.030.7143381
GCAGAGT37600.029.8622869
TAGGCCC1453.6238664E-529.8339214
ACATGGG19650.028.9862213
GTCTAGC1000.00622165528.8571911
GGCTATA1255.303537E-428.8571911
GGTATCA15600.027.2848451
TCTACAC3555.456968E-1226.4023193
CAGCGCT4500.025.5913859
GTATGTA9650.025.4182531
AGCGCTG4300.025.1506885
Produced by FastQC (version 0.11.5)