FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences733458
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16470.22455273512593768No Hit
GTAATAAACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG15630.2131001366131394No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12260.1671534021034606No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11760.16033637917917593No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11260.15351935625489121No Hit
GTTTATTACTGTGCGAGGAAATATTGTACTACTTCCTATTGCCCAGGTCA11110.1514742493776058No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10870.14820207837394916No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGTA10590.14438454553634975No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10320.140703353157236No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10200.13906726765540767No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10080.13743118215357933No Hit
GTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGT9980.1360677775687224No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9510.1296597760198948No Hit
ACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTTTATTACTGT8830.12038862484286762No Hit
CCCCTGGACAGGGGCCTGAGTGGATGGGAGGGATCGTCCCTTTCTTTGAT8660.11807083704861084No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8410.11466232558646847No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8040.1096177286224978No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7830.10675457899429824No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7580.1033460675321559No Hit
GCCTACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTTTATTA7460.10170998203032756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC10800.063.8872874
ATCAACG11150.062.5264553
CAACGCA12050.057.263895
CGCAGAG12750.053.6625678
AACGCAG13000.053.0538446
ACGCAGA17500.039.920117
GTATCAA19450.038.4729651
TATCAAC21950.033.089582
CAGCGCT3500.032.9237989
GTATAGA2101.5490514E-830.8365061
TACACGG7200.029.9348966
GTACACG7500.028.7512425
TACGACC1050.00804066927.3802644
ACACGGG8050.026.8400557
AGCCGAT1358.399721E-426.6743767
GTATGTA9750.026.5668371
ACGGGGT7900.026.4380179
GTACATG18900.026.2681351
ACATGGG18900.025.8591383
TATGTAC8150.025.5884972
Produced by FastQC (version 0.11.5)