FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155676
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGCTACTACTGGGGCTGGATCCGGCA53400.4620672229932957No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA47220.4085920275232851No Hit
GTATATTACTGTGCGCGAGTCCGGTATTACGATTTTTGGAGTGGTTATTC32680.28277821811649634No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT27750.24011920295999917No Hit
ATATTACTGTGCGCGAGTCCGGTATTACGATTTTTGGAGTGGTTATTCCC26210.22679366881375057No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26100.22584184494616136No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC24730.21398731132255064No Hit
CTACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG23770.20568048484177226No Hit
GTATTACTGTCAACAGTATGATAATTCACCGCTCACTTTCGGCGGAGGGA23530.20360377822157766No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23350.20204624825643172No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21930.18975906742028043No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20530.17764494546914533No Hit
GTCTATCATGGTGGGAGCACCTACTACAACCCGTCCCTGGAGAGTCGAGT20400.17652006271653992No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20320.17582782717647508No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19770.17106870783852912No Hit
TCCCTGGAGAGTCGAGTCAGCATATCAGCAGACACGTCCAAGAACCAGTT19440.16821323623576157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19390.16778058902322104No Hit
CAGTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA19350.1674344712531886No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT18750.16224270470270213No Hit
ACTACAACCCGTCCCTGGAGAGTCGAGTCAGCATATCAGCAGACACGTCC18680.16163699860514538No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC18430.1594737625424427No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGCAGTGGCTACTACTGGGGCT18050.1561856437271346No Hit
ATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACT17400.1505612299641076No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17300.14969593553902652No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16820.14554252229863732No Hit
GCCGTATATTACTGTGCGCGAGTCCGGTATTACGATTTTTGGAGTGGTTA16290.14095646184570762No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16000.1384471080129725No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15220.13169781149734008No Hit
AACCAGTAGAGGGCGGGGGAATAACCACTCCAAAAATCGTAATACCGGAC14990.1297076343196536No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14720.1273713393719347No Hit
GAGTATTAGCAGCTACTTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTC14440.12494851498170767No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13970.12088163118382661No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13650.11811268902356717No Hit
GTATGATAATTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA13430.11620904128838878No Hit
GTGTATTACTGTCAACAGTATGATAATTCACCGCTCACTTTCGGCGGAGG13420.11612251184588068No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13220.11439192299571851No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13120.11352662857063743No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12950.11205562804799962No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12710.10997892142780502No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12660.10954627421526449No Hit
CAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGCTGAGAGT12640.10937321533024827No Hit
GGCTACTACTGGGGCTGGATCCGGCAGTCCCCAGGGAAGGGGCTGGAGTG12300.1064312142849726No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12230.10582550818741585No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12090.10461409599230233No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12080.10452756654979424No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC11850.10253738937210777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)