FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155676
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTTACTCCATCAGCAGTGGCTACTACTGGGGCTGGATCCGGCA49960.4323010947705066No Hit
GTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA44530.3853156074886041No Hit
GTATATTACTGTGCGCGAGTCCGGTATTACGATTTTTGGAGTGGTTATTC32330.27974968762871255No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26790.23181237647922084No Hit
ATATTACTGTGCGCGAGTCCGGTATTACGATTTTTGGAGTGGTTATTCCC24120.20870901532955605No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23870.20654577926685333No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22930.1984120116710912No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21860.18915336132272367No Hit
GTATTACTGTCAACAGTATGATAATTCACCGCTCACTTTCGGCGGAGGGA21730.18802847857011826No Hit
CTACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG20120.1740972383263129No Hit
GTCTATCATGGTGGGAGCACCTACTACAACCCGTCCCTGGAGAGTCGAGT19730.17072259006849672No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19440.16821323623576157No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18930.16380023466784807No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18900.16354064634032375No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18770.16241576358771836No Hit
CAGTAATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA18710.16189658693266973No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18350.15878152700237783No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT17400.1505612299641076No Hit
TCCCTGGAGAGTCGAGTCAGCATATCAGCAGACACGTCCAAGAACCAGTT16780.1451964045286049No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC15840.13706263693284276No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15840.13706263693284276No Hit
ATATACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACT15490.134034106445059No Hit
ACTACAACCCGTCCCTGGAGAGTCGAGTCAGCATATCAGCAGACACGTCC15470.13386104756004277No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15460.13377451811753466No Hit
CACCTGCACTGTCTCTGGTTACTCCATCAGCAGTGGCTACTACTGGGGCT15260.13204392926737252No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15030.13005375208968603No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14350.12416974999913472No Hit
AACCAGTAGAGGGCGGGGGAATAACCACTCCAAAAATCGTAATACCGGAC14090.12191998449392391No Hit
GTGTATTACTGTCAACAGTATGATAATTCACCGCTCACTTTCGGCGGAGG13940.12062204285630228No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13930.12053551341379418No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13870.12001633675874553No Hit
GCCGTATATTACTGTGCGCGAGTCCGGTATTACGATTTTTGGAGTGGTTA13800.11941063066118877No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13750.11897798344864823No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13290.11499762909327528No Hit
GTATGATAATTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA13080.11318051080060501No Hit
GAGTATTAGCAGCTACTTAGCCTGGTACCAGCAGAAAGCTGGCCAGGCTC13040.11283439303057259No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12850.11119033362291854No Hit
CAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGCTGAGAGT12440.1076426264800861No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12420.1074695675950699No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12340.10677733205500504No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12310.10651774372748071No Hit
GGCTACTACTGGGGCTGGATCCGGCAGTCCCCAGGGAAGGGGCTGGAGTG11920.10314309546966452No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11780.101931683274551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)