FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984304
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29870.30346315772363014No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23870.24250638014272013No Hit
ATTTATTACTGTGTGTGTTCTGGTGGGAGCTACTCAGACTACTGGGGCCA19240.19546806677611794No Hit
GTAATAAATAGCCGTGTCCTCGGCTCTCAGGTTGTCCATTTGCAGATACA16640.16905346315772363No Hit
ATATTACTGTCAACACTATCATGGTCTCCCTCGGACTTTCGGCGGAGGGA16540.16803751686470847No Hit
ATCATGGTCTCCCTCGGACTTTCGGCGGAGGGACCAAGGTGGATATGAAA15080.15320470098668704No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14980.15218875469367188No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14780.15015686210764154No Hit
TTATTACTGTGTGTGTTCTGGTGGGAGCTACTCAGACTACTGGGGCCAGG14430.14660105008208846No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13650.13867666899657016No Hit
GCATGGACATGAGGGTCCCAGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13380.13593361400542922No Hit
CTATCATGGTCTCCCTCGGACTTTCGGCGGAGGGACCAAGGTGGATATGA12330.12526617792876996No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12120.12313269071343813No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11960.12150717664461386No Hit
CAGTAATAAATAGCCGTGTCCTCGGCTCTCAGGTTGTCCATTTGCAGATA11720.11906890554137747No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11640.11825614850696532No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11410.11591947203303044No Hit
CTATTTAAGTTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTCA11020.11195728149027129No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10940.11114452445585916No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10780.10951901038703489No Hit
TCTATAGGAGACGAAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAA10750.10921422649913036No Hit
CCCCTAAACTCCTCATCTACGATGCATCCAATTTAAAGACAGGGGTCCCA10540.1070807392837985No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10480.1064711715079894No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10340.10504884669776816No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10320.10484565743916513No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10250.10413449503405452No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10240.104032900404753No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACGAAGTCACCATCACTT10220.10382971114614996No Hit
TATAAAAGGTGTCCACTGTCAGGTTCAACTGGTGGAGTCTGGGGGAGGCT10130.10291535948243632No Hit
ACATATTACTGTCAACACTATCATGGTCTCCCTCGGACTTTCGGCGGAGG9990.10149303467221509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)