FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073390
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCTTCAGATCTCAGGCTGCCCAATTCCATGAAAA43380.40414015409124365No Hit
ATATATTACTGTGCGAGAGATATGGAGTGTCGTGGTACCATATGTTATAG42980.400413642757991No Hit
ATGTTATAGCGGTGCTTTTGATATATGGGGCCAGGGGACAGTGGTCACCG29680.27650714092734235No Hit
CTATAACATATGGTACCACGACACTCCATATCTCTCGCACAGTAATATAT24120.22470863339513128No Hit
GTAATAATCACCTTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG22460.20924361136213307No Hit
GCCATATATTACTGTGCGAGAGATATGGAGTGTCGTGGTACCATATGTTA21090.19648031004574296No Hit
GATTATTACTGTGCAGCTTGGGATGACAGCCTGATTGGTTTTGTCTTCGG20660.19247431036249638No Hit
ATATTACTGTGCGAGAGATATGGAGTGTCGTGGTACCATATGTTATAGCG19050.1774751022461547No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18550.17281696307958896No Hit
GTGCAGGGTCCTGGGCCCAATCTGTGGTGACTCAGCCACCCTCAGCGTCT18270.17020840514631214No Hit
ATATATGGCCGTGTCTTCAGATCTCAGGCTGCCCAATTCCATGAAAATTG17800.16582975432974037No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17700.1648981264964272No Hit
GTCCGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGTA16950.1579109177465786No Hit
CCTCATTCACTGTGCAGGGTCCTGGGCCCAATCTGTGGTGACTCAGCCAC16840.15688612712993413No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16780.15632715042994624No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16100.14999208116341683No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16010.149153616113435No Hit
CCTGTAAGGCCTCTGGACTCAGCCTCAGCAACTCTGCTATCGCCTGGGTG15970.14878096498010973No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15470.144122825813544No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA15460.1440296630302127No Hit
GATATGGAGTGTCGTGGTACCATATGTTATAGCGGTGCTTTTGATATATG15320.14272538406357427No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG15260.14216640736358638No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT14200.132291152330467No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT14120.1315458500638165No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13540.12614240863060025No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13480.12558343193061236No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13450.12530394358061842No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13370.1245586413139679No Hit
CAGTAATATATGGCCGTGTCTTCAGATCTCAGGCTGCCCAATTCCATGAA13080.12185692059735977No Hit
CTCCATATCTCTCGCACAGTAATATATGGCCGTGTCTTCAGATCTCAGGC12790.11915519988075164No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG12740.11868938596409508No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12680.11813040926410717No Hit
GGTATCACCAGCTCCCAGGAGCGGCCCCCAAACTCCTCATCTATAACAAT12570.11710561864746272No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12390.11542868854749905No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12240.11403124679752932No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12220.11384492123086669No Hit
TCCTTGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC12160.11328594453087881No Hit
ACAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTTTCTGGCTCCAAG12040.11216799113090302No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC11930.11114320051425856No Hit
GTCCTGGGCCCAATCTGTGGTGACTCAGCCACCCTCAGCGTCTGAGACCC11900.11086371216426462No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11770.10965259598095753No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.10927994484763227No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11680.1088141309309757No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG11400.10620557299769887No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT11160.10396966619774732No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC11150.103876503414416No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11010.10257222444777761No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10900.10154743383113314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18850.070.777221
TCAACGC20700.063.6776544
ATCAACG21000.062.4249763
CAACGCA21450.060.779565
AACGCAG22700.057.381836
TATCAAC23300.056.571992
ACGCAGA25700.050.676487
CGCAGAG26100.049.8998268
GTACATG26400.039.062971
TACATGG27500.036.931142
GCAGAGT37450.034.776659
CATGGGG13450.033.2028774
GTATTAG3301.8189894E-1228.4094331
CAGAGTA30150.027.684189
AGGGGTG10750.026.1313845
GAGTTTA5600.025.7558041
GGTATCA26550.025.261061
ACATGGG40850.024.5091933
CTTATAG3551.6734703E-1024.3477692
ATATTAC24950.023.6727983
Produced by FastQC (version 0.11.5)