FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525690
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23970.45597215088740517No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGACTCACTTTCAGTAGCGCCTGG21210.4034697255036238No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17480.33251536076394833No Hit
GTAATACACGGCTGTGTCGTCGGTTGTCAGGCTGCTCATTTGCAGATACA16170.3075957313245449No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15920.30284007685137626No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15900.3024596244935228No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15890.30226939831459604No Hit
GAGTACGCTGCGCCCGTGGAAGACAGATTCACCATCTCAAGAGATGATTC15100.28724153017938325No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14600.27773022123304614No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14500.27582795944377864No Hit
GCTCTAGTACCATCAGTCTTGCTTGTAATAAGGCCAACCCACTCCAGCCC12540.23854362837413684No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12470.23721204512164967No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA12300.23397820007989498No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12150.23112480739599386No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11560.21990146283931594No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11520.21914055812360897No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11470.21818942722897525No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10870.2067758564933706No Hit
GTGTATCTGCAAATGAGCAGCCTGACAACCGACGACACAGCCGTGTATTA10850.20639540413551713No Hit
GCGTACTCTGCTCTAGTACCATCAGTCTTGCTTGTAATAAGGCCAACCCA10850.20639540413551713No Hit
ATACACGGCTGTGTCGTCGGTTGTCAGGCTGCTCATTTGCAGATACACCG10820.20582472559873688No Hit
TTTAAAAGGTGTCCATTGTGAGGTGCTTCTGGTGGAGTCTGGGGGAGGCT10770.20487359470410318No Hit
CTCCTGTGCAGCCTCTGGACTCACTTTCAGTAGCGCCTGGATGATCTGGG10680.2031615590937625No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10260.19517205957883924No Hit
GTACTAGAGCAGAGTACGCTGCGCCCGTGGAAGACAGATTCACCATCTCA10180.1936502501474253No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10120.19250889307386482No Hit
GTGTATTACTGTTCCACCCCACCCCGCGTTGAGGGAATAATTATTCGCGA10100.19212844071601134No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10040.19098708364245087No Hit
GTCTAAGGGACCCCCCCGGCTTTACCAAGCCTCCCCCAGACTCCACCAGA10020.19060663128459737No Hit
GTATTACTGTTCCACCCCACCCCGCGTTGAGGGAATAATTATTCGCGACT9640.18337803648538112No Hit
TACTAGAGCAGAGTACGCTGCGCCCGTGGAAGACAGATTCACCATCTCAA9420.17919306054899276No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9120.17348627518119045No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9050.17215469192870322No Hit
GCCGTGTATTACTGTTCCACCCCACCCCGCGTTGAGGGAATAATTATTCG8800.16739903745553464No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8720.16587722802412067No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC8590.163404287698073No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8570.16302383534021953No Hit
CAGATACACCGTGTTTTTTGAATCATCTCTTGAGATGGTGAATCTGTCTT8410.15998021647739163No Hit
GTCTGGGGGAGGCTTGGTAAAGCCGGGGGGGTCCCTTAGACTCTCCTGTG8340.1586486332249044No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8330.15845840704597766No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8290.1576975023302707No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8190.15579524054100327No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7970.1516102646046149No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7900.15027868135212766No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7890.15008845517320094No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7860.1495177766364207No Hit
CAGTAATACACGGCTGTGTCGTCGGTTGTCAGGCTGCTCATTTGCAGATA7770.14780574102608No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC7690.14628393159466607No Hit
GTTCCACCCCACCCCGCGTTGAGGGAATAATTATTCGCGACTACTGGGGC7590.14438166980539863No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7590.14438166980539863No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7570.14400121744754513No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7530.14324031273183815No Hit
TTACTGTTCCACCCCACCCCGCGTTGAGGGAATAATTATTCGCGACTACT7490.14247940801613118No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATCCCCAAGTGT7320.13924556297437654No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7190.13677262264832887No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7180.13658239646940212No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT7070.13448990850120793No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT7000.13315832524872073No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6830.1299244802069661No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT6790.12916357549125912No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT6740.1282124445966254No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG6720.1278319922387719No Hit
CTATTACTGTCAGCACTATGGTAACTCACACCTCTCTTTCGGCGGAGGGA6710.12764176605984515No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG6700.12745153988091842No Hit
GCCTTATTACAAGCAAGACTGATGGTACTAGAGCAGAGTACGCTGCGCCC6520.12402746866023702No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC6330.12041317126062889No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA6280.11946204036599518No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6250.11889136182921495No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6210.11813045711350795No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC6200.11794023093458121No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA6190.11775000475565447No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA6170.11736955239780099No Hit
GAACAGTAATACACGGCTGTGTCGTCGGTTGTCAGGCTGCTCATTTGCAG6150.11698910003994749No Hit
CACTTTCAGTAGCGCCTGGATGATCTGGGTCCGCCAGGCTCCAGGGAAGG6050.11508683825068006No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA6040.11489661207175332No Hit
TCTCTTGAGATGGTGAATCTGTCTTCCACGGGCGCAGCGTACTCTGCTCT5990.11394548117711958No Hit
GTCTATTACTGTCAGCACTATGGTAACTCACACCTCTCTTTCGGCGGAGG5900.11223344556677892No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA5880.11185299320892542No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC5840.11109208849321844No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGC5820.11071163613536494No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT5780.10995073141965797No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC5760.10957027906180448No Hit
AGAGTACGCTGCGCCCGTGGAAGACAGATTCACCATCTCAAGAGATGATT5700.10842892198824401No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTCCTTGC5700.10842892198824401No Hit
GCCTTGGGATCCCCAAGTGTTTTCATTCAGTGATCAGGACTGAACACAGA5670.10785824345146379No Hit
GGGTGGGGTGGAACAGTAATACACGGCTGTGTCGTCGGTTGTCAGGCTGC5640.10728756491468355No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCT5570.10595598166219634No Hit
AGACTCTCCTGTGCAGCCTCTGGACTCACTTTCAGTAGCGCCTGGATGAT5520.10500485076756264No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5510.10481462458863589No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGG5470.10405371987292891No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT5450.10367326751507541No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC5450.10367326751507541No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC5430.10329281515722194No Hit
ATGGTAACTCACACCTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAG5350.10177100572580798No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGATCATCCAGGCGCTAC5280.10043942247332077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)