FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459915
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG14210.30897013578596044No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA12950.2815737690660231No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11580.25178565604513875No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11300.24569757455181937No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10450.22721589858995683No Hit
GTGTATTACTGTACCACAGGTTTAGTACCAGCGGACAACTGGTTCGACCC10260.22308470043377582No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGG9760.21221312633856257No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9690.2106911059652327No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT9450.20547275039953036No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9170.1993846689062109No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG8870.19286172444908298No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT8600.18699107443766783No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8480.18438189665481663No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8310.18068556146244413No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC8300.18046812998053988No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC8170.17764152071578443No Hit
ATACCACACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC8090.17590206886055032No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT7890.17155343922246502No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7750.1685093984758053No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA7650.16633508365676267No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7600.16524792624724136No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG7400.16089929660915603No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG6860.14915799658632573No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA6860.14915799658632573No Hit
TTACTGTACCACAGGTTTAGTACCAGCGGACAACTGGTTCGACCCCTGGG6850.14894056510442147No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT6800.14785340769490016No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6750.14676625028537882No Hit
ATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACAGCG6730.14633138732157028No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6650.14459193546633617No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGAGCTGGG6610.14372220953871911No Hit
GTACTAAACCTGTGGTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGG6590.14328734657491057No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCT6540.14220018916538926No Hit
GTCTAAGGGACCCCCCAGGCTTTACCAAGCCTCCCCCAGACTCCACCAGC6510.14154789471967646No Hit
GTATTACTGTACCACAGGTTTAGTACCAGCGGACAACTGGTTCGACCCCT6460.14046073731015513No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6370.13850385397301676No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA6320.13741669656349545No Hit
TCCTATGACAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCT6280.13654697063587837No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6280.13654697063587837No Hit
GCCGTGTATTACTGTACCACAGGTTTAGTACCAGCGGACAACTGGTTCGA6230.13545981322635703No Hit
GTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAG6190.13459008729874No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGC6120.13306806692541012No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT6020.1308937521063675No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG6000.13045888914255896No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT6000.13045888914255896No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6000.13045888914255896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5980.13002402617875042No Hit
GTATCTGCAAATGAACAGCCTGAAAACCGAGGACACAGCCGTGTATTACT5730.1245882391311438No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG5650.12284878727590968No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG5640.12263135579400541No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT5630.12241392431210116No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC5580.12132676690257982No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG5470.1189350206016329No Hit
CTGTAGCAGCCACAGAGGTCACGGTCAGGTGGTCCTTGAACAGCGCTCTT5380.11697813726449452No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT5370.11676070578259026No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTAAAGCCTGGGG5270.11458639096354761No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG5260.11436895948164336No Hit
TGCAGATACAGCGTGTTTTTTGAATCATCTCTTGAGATGGTGAATCTGCC5180.11262950762640923No Hit
GGTTTAGTACCAGCGGACAACTGGTTCGACCCCTGGGGCCAGGGAACCCT5110.11110748725307938No Hit
GTGTACATCATAACCTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA5000.10871574095213246No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG4860.10567170020547274No Hit
CGCTGTAGCAGCCACAGAGGTCACGGTCAGGTGGTCCTTGAACAGCGCTC4730.10284509094071731No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA4660.10132307056738746No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4660.10132307056738746No Hit
GTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGC4640.10088820760357892No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4600.10001848167596186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)