FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459915
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGG13850.30114260243740687No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA12300.26744072274224584No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11310.24591500603372363No Hit
GTGTATTACTGTACCACAGGTTTAGTACCAGCGGACAACTGGTTCGACCC9960.21656175597664787No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC9150.1989498059424024No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGG8730.18981768370242327No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8720.18960025222051902No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA8610.18720850591957208No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8480.18438189665481663No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT8290.18025069849863562No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8160.17742408923388017No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8120.17655436330626312No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8030.17459747996912472No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC7760.16872682995770957No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT7740.16829196699390106No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7740.16829196699390106No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7690.16720480958437972No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7570.16459563180152853No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA7360.16002957068153897No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG7260.15785525586249632No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG7180.15611580400726222No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA7170.15589837252535796No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7150.15546350956154942No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT7080.15394148918821957No Hit
ATACCACACCACTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC7060.15350662622441105No Hit
TTACTGTACCACAGGTTTAGTACCAGCGGACAACTGGTTCGACCCCTGGG7040.1530717632606025No Hit
GTCTAAGGGACCCCCCAGGCTTTACCAAGCCTCCCCCAGACTCCACCAGC7000.15220203733298546No Hit
CTCCTGTGCAGCCTCTGGATTCACTTTCAGTAACGCCTGGATGAGCTGGG6660.14480936694824043No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC6650.14459193546633617No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC6560.1426350521291978No Hit
GTATTACTGTACCACAGGTTTAGTACCAGCGGACAACTGGTTCGACCCCT6340.13785155952730394No Hit
TCCTATGACAGCAGCCTGAGTGGTGTGGTATTCGGCGGAGGGACCAAGCT6250.13589467619016557No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCT6190.13459008729874No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG6120.13306806692541012No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT5950.12937173173303762No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG5950.12937173173303762No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG5930.1289368687692291No Hit
GTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAG5910.12850200580542057No Hit
ATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACAGCG5910.12850200580542057No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT5810.12632769098637792No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG5770.12545796505876086No Hit
CCCTCATGCTGCCACCCCCGACTGTCACTGCACCGACCGGCCCTCGAGGA5700.12393594468543101No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTAAAGCCTGGGG5550.12067447245686704No Hit
GCCGTGTATTACTGTACCACAGGTTTAGTACCAGCGGACAACTGGTTCGA5520.12002217801115424No Hit
GTACTAAACCTGTGGTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGG5480.11915245208353718No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC5480.11915245208353718No Hit
GGTTTAGTACCAGCGGACAACTGGTTCGACCCCTGGGGCCAGGGAACCCT5440.11828272615592011No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5430.11806529467401584No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC5380.11697813726449452No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC5320.11567354837306892No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT5290.11502125392735614No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG5280.11480382244545188No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAGGC5280.11480382244545188No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG5260.11436895948164336No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA5260.11436895948164336No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC5210.11328180207212202No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA5190.11284693910831349No Hit
GTATCTGCAAATGAACAGCCTGAAAACCGAGGACACAGCCGTGTATTACT5180.11262950762640923No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTC5180.11262950762640923No Hit
GGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC4920.10697628909689834No Hit
GTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCG4920.10697628909689834No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC4870.10588913168737703No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG4770.10371481686833436No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA4730.10284509094071731No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA4690.10197536501310024No Hit
TGCAGATACAGCGTGTTTTTTGAATCATCTCTTGAGATGGTGAATCTGCC4680.10175793353119598No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG4680.10175793353119598No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4670.10154050204929171No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG7250.044.706273
TCAACGC7300.044.4000664
GTATCAA7350.044.1028181
TATCAAC7850.042.2067682
CAACGCA8050.040.2634125
GTCTTAC1105.49584E-639.2916031
TCTACAC4650.038.7240683
ACGCAGA8400.038.5689777
AACGCAG8400.038.5689776
CGCAGAG8400.038.5689778
GTACCTA750.001530215138.4184571
GAATACG1502.7590431E-838.3975649
CTCTACA5000.034.572852
TAGACTA850.002829857433.894955
GCAGAGT9400.033.6999939
GTGCTAG1902.1927917E-730.330361
ACTGATC1204.2239964E-429.9980988
CAGTGAA5300.029.8848959
ACAGTGA5550.029.8359458
GTACATG11350.028.5599751
Produced by FastQC (version 0.11.5)