FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589064
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG15830.26873141118791843No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGG12270.2082965518177991No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG11640.19760161883937907No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11550.19607377127103337No Hit
ATGTATTACTGTGCGAGAATCGGGGGGAGCATCCCATCTTACTGGTACTT9930.1685725150408105No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGG9610.16314016813113685No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC9260.1571985386986813No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9060.15380332188013526No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT9040.15346380019828065No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG8890.15091738758437112No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG7810.13258321676422258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.1252835006043486No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7200.12222780546765717No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6490.11017478576181876No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6460.10966550323903684No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC6380.10830741651161842No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6370.10813765567069113No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6130.10406339548843589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14550.056.431071
TCAACGC14300.053.8691064
ATCAACG14700.052.4166263
CAACGCA14750.052.2256435
AACGCAG15150.050.846756
TATCAAC15400.050.0340582
CGCAGAG16800.045.852878
ACGCAGA18600.041.4154977
GCAGAGT19750.040.462059
GCCGAGT850.002831060733.893581
CAGCGCT2850.032.8390469
GTGATAC1153.2850407E-431.3147221
TGTACTA3700.031.1322355
CCGAGTG950.00488456230.3206882
TATAGCC1504.6030073E-528.8046533
GTGTAGA2603.983587E-927.7014851
ACTAGAC1100.00999220626.1860493
TACACGA1100.00999220626.1860492
GTATTAG2851.0595613E-825.271531
GTACATG16900.025.1444241
Produced by FastQC (version 0.11.5)