FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589064
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGG12930.219500767319001No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11760.1996387489305067No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG11510.19539472790732418No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC10490.1780791221327394No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG10450.1774000787690302No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT9830.16687490663153748No Hit
ATGTATTACTGTGCGAGAATCGGGGGGAGCATCCCATCTTACTGGTACTT9250.157028777857754No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG8840.15006858337973464No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG8730.1482012141295343No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGG8350.14175030217429685No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8110.13767604199204161No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG7010.11900234949003842No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6900.11713498023983812No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC6750.11458856762592859No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6710.1139095242622194No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6270.10644004726141812No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC6270.10644004726141812No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT750.001535304538.394296
GTATCAA9050.037.421661
TCAACGC8700.037.2516634
CTAAGAC1752.584784E-937.045093
ATCAACG8900.035.6113853
TACACGG3350.034.382956
CAACGCA9450.033.5302165
TATCAAC10100.032.0962452
AACGCAG9900.031.9952456
GTCCTAT2051.2068995E-831.634611
CGCAGAG10050.031.5177048
GTACACG3700.031.1410875
ACACGGG4000.028.795727
ACCTAGC1306.716363E-427.7140831
GTACATG15100.027.2001761
TCTACAC4300.026.8026473
ACGCAGA11850.026.7302047
GGACCTA1400.00103897225.7191985
GTATTAG2551.0051917E-725.4317461
TACGAGT2601.2239798E-724.9193766
Produced by FastQC (version 0.11.5)