FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767310
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA41140.5361587884948716No Hit
GTGTATTACTGTGCGAGAGATGGTCACCGCGGTGATTACTATGATAGTAG28360.3696028984373982No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21530.2805906348151334No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG20190.26312702818938893No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16040.20904197781861306No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15420.20096180161864174No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14920.19444553048963262No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC14660.19105706950254786No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA14580.1900144661219064No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14330.18675633055740185No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13740.17906713062517107No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13720.17880647978001069No Hit
GTATTTATTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT13450.17528769337034575No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12760.16629523921231315No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12590.16407970702845004No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12120.15795441216718145No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT11970.1559995308284787No Hit
CTATGATAGTAGTGGGTTCTTTGACTACTGGGGCCAGGGAACCCTGGTCA11650.15182911730591286No Hit
CTATCATAGTAATCACCGCGGTGACCATCTCTCGCACAGTAATACACAGC11520.1501348868123705No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11150.1453128461769037No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10950.14270633772530006No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG10810.14088178180917751No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA10720.13970885300595587No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10640.1386662496253144No Hit
GTATTACTGTGCGAGAGATGGTCACCGCGGTGATTACTATGATAGTAGTG10490.13671136828661165No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10460.1363203920188711No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10380.13527778863822967No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9780.1274582632834187No Hit
GTGCATAGCATAGCTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC9580.12485175483181504No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC9520.12406980229633395No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT9490.1236788260285934No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9370.1221149209576312No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTAC9370.1221149209576312No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGG9350.12185427011247084No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTAATAAATACTA9290.12107231757698975No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT8900.11598962609636262No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8760.11416507018024007No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8590.11194953799637695No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8530.11116758546089586No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT8510.1109069346157355No Hit
CTACTATCATAGTAATCACCGCGGTGACCATCTCTCGCACAGTAATACAC8490.11064628377057513No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC8360.10895205327703275No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8330.1085610770092922No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC8090.10543326686736781No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT8080.10530294144478765No Hit
GCTGTGTATTACTGTGCGAGAGATGGTCACCGCGGTGATTACTATGATAG8050.10491196517704708No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8030.10465131433188672No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC8010.10439066348672636No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7960.10373903637382544No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG7910.10308740926092454No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7870.1025661075706038No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7820.10191448045770288No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7760.10113252792222178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG2050.056.193249
ATCAACG8100.053.3385053
TCAACGC8600.050.2374274
CAACGCA8800.049.0956655
GTAGCGA450.00990672347.9983946
GGTATCA4350.046.3553621
AACGCAG9450.045.7127536
GTATCAA9650.045.5232961
GTGCTAG2550.045.186741
TATCAAC10300.041.9485472
CTAGAGA3850.041.1468434
CGCAGAG10900.040.292238
TATATCG750.001533743138.403725
GCTAGAG3700.036.9765553
TACATGG15900.035.326482
GTACATG16200.035.1190951
AGAATAC3650.033.5331238
ACATGGG16150.033.4397743
GCAGAGT13300.033.021459
GGCAGCG3950.032.809037
Produced by FastQC (version 0.11.5)