FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090336
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTATCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGA31890.2924786487834943No Hit
GTCTCTGGTTTCTCCATCAGCAGTGGTTTCTACTGGGGCTGGATCCGGCA28150.25817729580606347No Hit
GTGTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATTC25490.2337811463622223No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21680.19883778945205882No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21540.1975537815865935No Hit
CTACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG20260.1858142811023391No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18290.1677464561382913No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17830.1635275731517624No Hit
ACTACAACCCGTCCCTCCACAGTCGAATCACCATGTCAGTAGACACGTCC17750.1627938543714965No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17580.16123470196343145No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17480.16031755348809906No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17330.15894183077510052No Hit
GTATTACTGTCAGCAGTATGATGGCTCATCCACTTTCGGCGGAGGGACCA16080.14747747483344584No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15630.14335030669445015No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15600.14307516215185045No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15400.14124086520118567No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14730.1350959704164588No Hit
GTATTACTGTGCGAGAGCGAGATATTACGATTTTTGGAGTGGTTATTCCC13630.12500733718780266No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13030.11950444633580841No Hit
CACCTGCACAGTCTCTGGTTTCTCCATCAGCAGTGGTTTCTACTGGGGCT12850.11785357908021014No Hit
AACCAGTAGTGGGCCGGGGAATAACCACTCCAAAAATCGTAATATCTCGC12240.11225897338068265No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12200.11189211399054971No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11530.10574721920582279No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11510.10556378951075633No Hit
TCCCTCCACAGTCGAATCACCATGTCAGTAGACACGTCCAAGAACCAGTT11090.10171176591436035No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11080.1016200510668271No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8000.075.778741
GTATCAA21550.073.677261
ATCAACG24900.062.4790733
TCAACGC24900.062.1898164
CAACGCA26200.059.101355
TATCAAC27000.057.6195872
AACGCAG27650.056.2186166
CGCAGAG33100.046.962088
ACGCAGA37200.041.7861487
GCAGAGT40500.038.7367369
CAGCGCT8400.035.125799
GTACACG12550.030.4153215
GCAGCGC9800.030.1078198
GGCAGCG10800.028.6527467
GTACATG28950.028.1700731
ACGGGGT13650.027.9424679
GAATACG1555.7855534E-527.8574319
TAGCGTA1050.00799242127.4152497
ACACGGG13950.027.3415557
TACATGG29900.026.9790732
Produced by FastQC (version 0.11.5)