FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304506
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC75540.5790697781382378No Hit
TCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC38610.2959741082064782No Hit
CCCTATGATCACCACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA37720.28915160221570463No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA36100.2767331081650832No Hit
TCATAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC34670.2657711041574358No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32670.2504396300208661No Hit
GCCTTGGGCTGACCTATGACGGTCAGCTTGGTCCCTCCGCCAAACACCCT31520.24162403239233857No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT31220.23932431127185308No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG30410.23311506424654238No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA30120.23089200049673975No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28350.2173236458858756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26620.2040619207577428No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCC26310.20168554226657445No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG25840.19808264584448057No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25520.19562960998262946No Hit
ATCATAGGGTGTTTGGCGGAGGGACCAAGCTGACCGTCATAGGTCAGCCC25510.19555295261194658No Hit
GACCTATGACGGTCAGCTTGGTCCCTCCGCCAAACACCCTATGATCACCA24720.18949702032800156No Hit
GTGTATTACTGTGCGAGAGGTGTCTATAGCGACACCTACCCTGCGTACTG24200.18551083705249344No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23800.1824445422251795No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG23420.17953156213923124No Hit
GTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG23090.17700186890669725No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23060.1767718967946487No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG22540.17278571351914057No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA22220.17033267765728943No Hit
GTAGTAGTCAGCTTCATCATCCAGTTGGGCCCCACTGATAGTCAAGGTGG21790.16703641071792694No Hit
GTTCTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAG21710.16642315175246417No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21670.16611652226973275No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21570.16534994856290428No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA21520.16496666170949004No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC21450.1644300601147101No Hit
GCTCTGGAGATGCATTGCCAAAAAAGTATGCTTATTGGTACCAGCAGAAG21050.16136376528739615No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19960.15300811188296565No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19690.15093836287452875No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA19680.15086170550384592No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19400.14871529912472614No Hit
CCCCTGTTCTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCT19090.14633892063355786No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT18840.14442248636648664No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT18840.14442248636648664No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTGATCATAGGGTGTTTGG18780.14396254214238952No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17690.13560688873795904No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16920.12970427119537972No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC16740.12832443852308842No Hit
CAGTAGTAGTCAGCTTCATCATCCAGTTGGGCCCCACTGATAGTCAAGGT16660.12771117955762565No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16650.1276345221869428No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG16290.12487485684236024No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16250.12456822735962886No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC16010.1227284504632405No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTGATCATAGGGTGTTTGGCG16000.12265179309255765No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA15790.12104198830821783No Hit
GGGTAGGTGTCGCTATAGACACCTCTCGCACAGTAATACACGGCCGTGTC15610.11966215563592654No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15610.11966215563592654No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT15440.11835898033431813No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA15270.11705580503270971No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15140.11605925921383267No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14920.11437279705881001No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14880.11406616757607861No Hit
CTCCAGAGACAACGCCAAGAATTCACTGTATCTACAAATGAACAGCCTGA14590.11184310382627599No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC14580.11176644645559315No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14340.1099266695592048No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA14290.10954338270579055No Hit
ATCTACAAATGAACAGCCTGAGAGCCGAGGACACGGCCGTGTATTACTGT14260.109313410593742No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14220.1090067811110106No Hit
GGGCTGGAGTGGGTGGCCAACATGAACCAAGATGAAAGTGAGACAAATTA14100.10808689266281642No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG14000.10732031895598793No Hit
GTATTACTGTGCGAGAGGTGTCTATAGCGACACCTACCCTGCGTACTGGG13870.10632377313711092No Hit
GTACATGGGAGGTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTT13750.10540388468891673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.10494394046481964No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGGAGTTATTGGATGACCTGGG13570.10402405201662544No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA13310.1020309603788714No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC13240.10149435878409145No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG13220.10134104404272576No Hit
GTCTATAGCGACACCTACCCTGCGTACTGGGGCCAGGGAACCCTGGTCAC13180.10103441455999436No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA13140.10072778507726296No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13090.10034449822384872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9100.081.672141
GTATCAA31550.070.6703341
ATCAACG34350.064.4029853
TATCAAC34800.063.777262
TCAACGC34700.063.7533844
CAACGCA35850.061.70125
AACGCAG36700.060.408216
TACGACT553.317577E-452.4072884
ACGCAGA42700.051.915967
CGCAGAG43500.050.9611858
GCAGAGT48350.045.9981169
GAATACG5850.036.9099469
GTGCTAG6500.035.523411
GTGTAGA6450.034.6800771
GTACATG35800.034.6675761
TACATGG36550.033.91062
TATTGAG24400.032.187045
GCTAGAG7050.031.6859663
TTATTGA25150.031.2307834
CAGAGTA47200.030.6501149
Produced by FastQC (version 0.11.5)