FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1304506
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC73990.5671878856823962No Hit
CCCTATGATCACCACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA35230.2700639169156754No Hit
TCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC33660.25802870971846814No Hit
TCATAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC33090.2536592395895458No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGTAGATACA32860.25189612006384027No Hit
GCCTTGGGCTGACCTATGACGGTCAGCTTGGTCCCTCCGCCAAACACCCT31500.24147071765097286No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29780.22828564989352293No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29420.22552598454894038No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT29160.2235328929111863No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG28220.21632710006699854No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA27440.21034782515373637No Hit
ATCATAGGGTGTTTGGCGGAGGGACCAAGCTGACCGTCATAGGTCAGCCC25010.19172008407780416No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCC24810.1901869366641472No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG24240.18581746653522482No Hit
GTGTATTACTGTGCGAGAGGTGTCTATAGCGACACCTACCCTGCGTACTG23540.18045145058742543No Hit
GACCTATGACGGTCAGCTTGGTCCCTCCGCCAAACACCCTATGATCACCA23120.17723184101874578No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23040.176618582053283No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22410.17178916770026353No Hit
GTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG21350.1636634864078816No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG21140.16205368162354178No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA20990.16090382106329906No Hit
GTTCTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCTGAGAG20440.15668766567574238No Hit
GTAGTAGTCAGCTTCATCATCCAGTTGGGCCCCACTGATAGTCAAGGTGG20270.15538449037413396No Hit
GCTCTGGAGATGCATTGCCAAAAAAGTATGCTTATTGGTACCAGCAGAAG20180.15469457403798834No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20160.15454125929662263No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG19930.15277813977091712No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA19780.15162827921067437No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC19350.1483320122713119No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19130.14664555011628924No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19120.1465688927456064No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19000.1456490042974122No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18700.14334928317692675No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTGATCATAGGGTGTTTGG18270.14005301623756425No Hit
CCCCTGTTCTGGTCATCTATGAGGACAGTAAACGACCCTCCGGGATCCCT18110.13882649830663868No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18020.13813658197049306No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT17320.13277056602269366No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT17140.13139073335040236No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16750.12840109589377127No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA16530.12671463373874864No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16070.12318839468733757No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT15590.11950884089456085No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC15580.119432183523878No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15580.119432183523878No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15160.11621257395519836No Hit
CAGTAGTAGTCAGCTTCATCATCCAGTTGGGCCCCACTGATAGTCAAGGT14920.11437279705881001No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTGATCATAGGGTGTTTGGCG14800.11345290861061583No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14630.11214973330900739No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14550.1115364743435446No Hit
GGGTAGGTGTCGCTATAGACACCTCTCGCACAGTAATACACGGCCGTGTC14000.10732031895598793No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13800.10578717154233097No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC13790.10571051417164812No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG13780.10563385680096528No Hit
GTCTATAGCGACACCTACCCTGCGTACTGGGGCCAGGGAACCCTGGTCAC13620.1044073388700397No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13600.10425402412867399No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA13550.10387073727525975No Hit
CTCCAGAGACAACGCCAAGAATTCACTGTATCTACAAATGAACAGCCTGA13450.10310416356843126No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAA13270.10172433089614No Hit
GGGCTGGAGTGGGTGGCCAACATGAACCAAGATGAAAGTGAGACAAATTA13120.10057447033589727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20550.067.264631
GGTATCA7150.066.456161
TCAACGC21450.063.367264
ATCAACG21700.063.300053
CAACGCA22750.059.7531475
TATCAAC23050.059.592672
AACGCAG23900.056.8823666
ACGCAGA26000.051.7347077
CGCAGAG27450.049.003798
GCAGAGT30000.044.8384749
GAATACG5700.040.3836489
GTGCTAG6400.039.371821
GTACATG38450.035.5757981
TACATGG38350.034.3175352
TATTGAG25950.034.091665
TTATTGA27550.032.630134
GCTAGAG7250.031.7425923
CATGGGG29200.029.3085614
TCTACAC14200.028.8680133
ATTGAGG12800.028.6598536
Produced by FastQC (version 0.11.5)