FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439368
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCACTTCTCAGGCTGTTCATCTCCAGATATA23860.5430527484932903No Hit
GTCTATTACTGTGCGAAAGGGGCAAGGAATTACTATGATAGTAGGGGATA20620.4693104641211923No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10010.22782724276688335No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCACCTGGTGGAGTCTGGGGGAGGCG9850.22418564847690317No Hit
GTATGATAGTCTCCCGCTCACTTTCGGCGGTGGGACCCGGGTGGAGATCA8580.19528049380018572No Hit
ATATTACTGTCATCAGTATGATAGTCTCCCGCTCACTTTCGGCGGTGGGA7470.1700169334134484No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7070.160912947688498No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6630.15089856339105262No Hit
CTCCTGTGAAGCGTCTGGATTCACCTTCAGTGGCTATGGCATGCACTGGG5830.13269059194115185No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5530.12586260264743904No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5490.12495220407494402No Hit
TAGTAATTCCTTGCCCCTTTCGCACAGTAATAGACAGCCGTGTCGTCACT5380.12244860800058265No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC5360.12199340871433513No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5340.12153820942808762No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA5250.1194898126399738No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5200.11835181442435498No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA5150.11721381620873618No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG5090.11584821834999363No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA4880.11106862584439468No Hit
CTATCATACTGATGACAGTAATATGTTGCAACGTCTTCAGGCTGCAGGCT4880.11106862584439468No Hit
CAGTAATATGTTGCAACGTCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT4790.10902022905628084No Hit
GAATTACTATGATAGTAGGGGATACTACTTTGACATCTGGGGCCAGGGAA4710.10719943191129078No Hit
CCTGTGAAGCGTCTGGATTCACCTTCAGTGGCTATGGCATGCACTGGGTC4680.10651663298191949No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4660.10606143369567196No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG4620.10515103512317692No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG4610.10492343548005316No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4600.1046958358369294No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTCGAAGTCGGCGGTGTT4540.10333023797818686No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT4440.10105424154694925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)