FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951369
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG36380.3823963152047208No Hit
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCCTCTCAGCTCCATGTAGG35190.36988802452045416No Hit
GTCTATTACTGTGCGGATCTGGACTGGAACGCCTCTGTCCCCCTCCATTT25860.27181882108834743No Hit
GATTATTACTGTGCAGCATGGGATGGCAGCCTGAGTGGTCGGGTGTTCGG25630.26940125230063205No Hit
CCTGTATACTGGTACCAGCACCTCCCAGGAACGGCCCCCAAACTCCTCAT21100.22178565835128117No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG19440.20433711840516142No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGCGTCT19080.2005530976939547No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGCGTCTGGGACCC18880.19845086396550654No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGAGTCCTCGGTGAAGGTCTCCTGCA18630.19582307180494632No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17430.18320966943425737No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17420.18310455774783496No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16850.1771131916217577No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16780.17637740981680086No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16000.16817869827585302No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15830.16639179960667205No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14930.15693174782865532No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14860.15619596602369848No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14840.15598574265085366No Hit
ACCTACATGGAGCTGAGAGGCCTGAGATCTGACGACACGGCCGTCTATTA14740.1549346257866296No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14430.15167616350753493No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13930.14642057918641455No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13820.14526435063576804No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12530.1317049430872774No Hit
TCTATAGGGATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGC12210.12834136912176033No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12110.12729025225753626No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12010.12623913539331216No Hit
GTGCTGACTCAGCCACCCTCGGCGTCTGGGACCCCCGGGCAGAGGGTCAC12010.12623913539331216No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT11770.12371645491917438No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11760.12361134323275196No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC11750.12350623154632956No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11740.12340111985990714No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11630.12224489130926067No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11590.12182444456357103No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11540.12129888613145899No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGGGATACTCAGCGGCCCT11200.11772508879309711No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11200.11772508879309711No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11170.11740975373382989No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11020.11583307843749376No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10890.11446662651400244No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10890.11446662651400244No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACTCAGG10740.11288995121766633No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10710.11257461615839912No Hit
GGGATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10610.11152349929417502No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10430.10963148893857166No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC10370.10900081882003723No Hit
CATCTATAGGGATACTCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG10260.10784459026939074No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG10240.10763436689654592No Hit
GTCCTTGGTAGAGCGAACTACGCACAGAAGTTCCAGGGCAGAGTCACGAT10120.10637302665947702No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10090.10605769160020981No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10000.10511168642240813No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9760.10258900594827033No Hit
GTATACTGGTACCAGCACCTCCCAGGAACGGCCCCCAAACTCCTCATCTA9600.1009072189655118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14200.078.756741
GGTATCA6050.066.7847061
ATCAACG18250.059.6130183
TCAACGC18350.058.895524
GTATACG502.058663E-457.7210661
CAACGCA19150.056.435135
TATCAAC19800.055.310232
AACGCAG20350.053.4107326
CGCAGAG23300.046.6459738
TAGACGG13250.046.2200135
AGACGGC13600.044.458666
ATAGACG13900.043.540314
ACGCAGA25800.041.847047
GACGGCC14300.041.7768027
GTAATAG15750.039.3969231
AATAGAC16250.038.1304663
TAATAGA16200.037.358662
CAGCGCT5000.034.5489129
GTACATG24800.034.039141
ACATGGG26800.031.1853223
Produced by FastQC (version 0.11.5)