FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130667
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA43690.3864090841954351No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT41920.3707546076784765No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35570.3145930676317607No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30720.2716980331078912No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30440.26922161874362655No Hit
ATAATACACGGCCGTGTCCTCAGACCTCAGGCTGCTCAGCTCCATGTAGG29600.26179237565083263No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29390.2599350648776342No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT28560.25259426515499256No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28050.24808365327722487No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27070.23941620300229863No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25290.22367328311518778No Hit
GCCTACATGGAGCTGAGCAGCCTGAGGTCTGAGGACACGGCCGTGTATTA23230.20545394886381224No Hit
GTATTATTGTGCGAGAGGGGGTAATTCGGGGCGTCTTTATAACGTCTGGG22540.19935135632330298No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAGCAG22290.19714027206949528No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG22160.19599050825751524No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21900.19369098063355525No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG21740.1922758867111183No Hit
CAATAATACACGGCCGTGTCCTCAGACCTCAGGCTGCTCAGCTCCATGTA20990.18564263394969518No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20050.1773289571553782No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG19240.17016504417304124No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT18850.16671575273710118No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG18820.16645042262664428No Hit
GTGTATTATTGTGCGAGAGGGGGTAATTCGGGGCGTCTTTATAACGTCTG18750.1658313190355781No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA18340.16220514085933346No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18330.16211669748918117No Hit
TCTTTATAACGTCTGGGGCTTCTGGGGCCAGGGAACCCTGGTCACCGTCT17550.15521811461730112No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17540.1551296712471488No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG17510.15486434113669187No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17240.15247637014257956No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17190.15203415329181802No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG16820.1487617485961826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16470.14566623064085182No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT16290.14407424997811027No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16020.14168627898399794No Hit
ACATGGAGCTGAGCAGCCTGAGGTCTGAGGACACGGCCGTGTATTATTGT15890.14053651517201793No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15720.1390329778794287No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC15720.1390329778794287No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15510.13717566710623022No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15440.13655656351516404No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15320.13549524307333638No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15220.13461080937181327No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG15190.13434547926135634No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGG14930.13204595163739632No Hit
AACCAGGGAAGGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAA14830.13116151793587325No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14570.12886199031191323No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14470.12797755661039015No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC14330.12673934942825782No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14220.12576647235658245No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT14150.12514736876551627No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14060.12435137843414552No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13960.12346694473262243No Hit
CATGTAGGCCGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT13720.12134430384896702No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13490.11931010633546392No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC13400.11851411600409317No Hit
ATACACGGCCGTGTCCTCAGACCTCAGGCTGCTCAGCTCCATGTAGGCCG13350.11807189915333163No Hit
TTATAAAGACGCCCCGAATTACCCCCTCTCGCACAATAATACACGGCCGT13330.11789501241302701No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13310.11771812567272238No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT13200.116745248601047No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13040.11533015467861006No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG12890.11400350412632543No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC12870.11382661738602082No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12850.1136497306457162No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12700.11232308009343159No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT12690.11223463672327928No Hit
CCATGTAGGCCGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCC12640.11179241987251773No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC12610.11152708976206081No Hit
ACTTACTACTGTCAACAGAGTTACGGTCCCCCCACTTTTGGCCAGGGGAC12350.10922756213810078No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG12230.10816624169627308No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12110.10710492125444537No Hit
AGCTACAGGTGTCCAGTCCCAGGTGCAGCTCGTGCAGTCTGGGGCTGAGG12030.10639737429322693No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11980.10595515744246538No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11960.10577827070216077No Hit
GTCTCCATCTTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTT11880.1050707237409423No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11860.10489383700063767No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA11780.1041862900394192No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA11710.10356718644835305No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11650.1030365262274392No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT11650.1030365262274392No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA11610.10268275274682996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18900.074.026481
ATCAACG21650.064.2591553
TATCAAC22200.062.6671452
TCAACGC22250.062.2023544
CAACGCA22800.061.018015
AACGCAG23850.058.569086
CGCAGAG26900.051.9122738
CAGCGCT7700.050.48059
ACGCAGA33800.041.3147967
GCAGCGC9200.040.685178
GTACATG28250.038.037771
ACATGGG29250.036.7194373
GGCAGCG10200.036.6964267
CATGGGA17000.033.9216274
CATGGGG14250.032.8801734
TACACCG1402.8572676E-530.8929085
GCAGAGT51200.028.3989329
GTCTTAG2105.2227006E-727.4737471
TCTACAC5250.027.4603633
CCTATAC1607.1020695E-527.0312963
Produced by FastQC (version 0.11.5)