FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130667
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT38570.34112607867745326No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA38350.33918032453410246No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT34100.3015918922193714No Hit
ATAATACACGGCCGTGTCCTCAGACCTCAGGCTGCTCAGCTCCATGTAGG29650.2622345925015942No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27620.2442805883606756No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT27300.24145040051580172No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27090.23959308974260324No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25300.2237617264853401No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23780.2103183342221892No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC23460.20748814637731533No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23190.205100175383203No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20760.18360843643619207No Hit
GTATTATTGTGCGAGAGGGGGTAATTCGGGGCGTCTTTATAACGTCTGGG20630.18245867262421206No Hit
GCCTACATGGAGCTGAGCAGCCTGAGGTCTGAGGACACGGCCGTGTATTA19780.17494098616126588No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG19700.1742334392000474No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGAGCATTAGCAG19420.17175702483578276No Hit
CAATAATACACGGCCGTGTCCTCAGACCTCAGGCTGCTCAGCTCCATGTA19380.17140325135517354No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG18030.1594633963846119No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17560.1553065579874534No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA17550.15521811461730112No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17380.15371457732471186No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17330.15327236047395035No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17120.15141504970075187No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17080.15106127622014262No Hit
GTGTATTATTGTGCGAGAGGGGGTAATTCGGGGCGTCTTTATAACGTCTG16820.1487617485961826No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG16350.1446049101990241No Hit
TCTTTATAACGTCTGGGGCTTCTGGGGCCAGGGAACCCTGGTCACCGTCT15790.13965208147049485No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15780.13956363810034256No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15740.1392098646197333No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG15730.139121421249581No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG15430.13646812014501175No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG14500.12824288672084708No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14490.12815444335069476No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT14270.126208689207344No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14190.12550114224612552No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14160.1252358121356686No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT14140.12505892539536398No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14040.1241744916938409No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13750.12160963395942395No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGG13740.12152119058927165No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13670.12090208699820548No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13600.12028298340713932No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC13460.119044776225007No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13420.11869100274439777No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13120.11603770163982853No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13070.11559548478906698No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC13030.11524171130845776No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12980.1147994944576962No Hit
CATGTAGGCCGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT12840.11356128727556389No Hit
TTATAAAGACGCCCCGAATTACCCCCTCTCGCACAATAATACACGGCCGT12820.11338440053525926No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12720.1124999668337362No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12590.11135020302175619No Hit
ACATGGAGCTGAGCAGCCTGAGGTCTGAGGACACGGCCGTGTATTATTGT12590.11135020302175619No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12490.11046576932023311No Hit
ATACACGGCCGTGTCCTCAGACCTCAGGCTGCTCAGCTCCATGTAGGCCG12470.11028888257992848No Hit
CCATGTAGGCCGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCC12330.10905067539779617No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG12260.10843157180673No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11750.10392095992896229No Hit
AACCAGGGAAGGCCCCTAAGCTCCTGATCTATGCTGCATCCAGTTTGCAA11710.10356718644835305No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11500.10170987567515456No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11360.10047166849302226No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11320.10011789501241303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11450.067.8512341
ATCAACG12500.060.4011963
TCAACGC12800.058.9881554
TATCAAC13200.057.7479632
CAACGCA13250.056.4420785
AACGCAG14250.052.448696
CGCAGAG17250.043.41378
CAGCGCT8750.040.3246469
GGCAGCG9300.037.1655737
GAATACG1051.9294953E-434.289679
GTACATG25050.033.885491
GTCCTAG2950.031.7001151
GTATGTA16900.031.4981121
ACGCAGA23950.031.2687387
GTACACG16000.031.0109185
GCAGCGC11400.030.9509358
ACATGGG25700.030.7769663
TATGTAC16250.030.5351812
ACACGGG17150.028.9712687
ACGGGGT18000.028.8033229
Produced by FastQC (version 0.11.5)