FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014481
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG42290.4168634010888326No Hit
GATTATTACTGTGCAGCATGGGATGACCGATGGGGTGGTCGACTGTTCGG31080.3063635494405514No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30470.3003506226336422No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC27640.27245458515240795No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26240.25865442526769844No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA25890.2552043852965211No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACAGTCGACCACCCCAT25660.2529372161726045No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24800.24445997510056863No Hit
ATAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTGGG24790.24436140252996358No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23810.2347012906106669No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT22900.22573118668560577No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22270.21952111473748648No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22220.21902825188446112No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21860.2154796393426787No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21730.2141981959248128No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACAGTCG20360.20069375375191847No Hit
GTCTATTATTGTGCGAGAGACTGGGTATCAGAACATGCCCCCTACGGAAT19890.1960608429334803No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19440.19162507725625222No Hit
CCCCTGGACAGGGGCTTGTGTGGATGGGAGGGATCTTCCCTTTGGAAACC19140.18866790013810017No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC18190.1793035059306187No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17680.17427630482976025No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG17160.1691505311582967No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17120.16875624087587643No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17120.16875624087587643No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16860.16619335404014468No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG16500.16264474149836222No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16120.15889898381536963No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16010.1578146855387139No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15760.15535037127358717No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15430.15209747644361996No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC15070.1485488639018375No Hit
TATGTATACTGGTACCAACAATTCCCAGGAACGGCCCCCAAACTCCTCAT14900.14687313020155132No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14870.14657741248973613No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14780.1456902593542905No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA14780.1456902593542905No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTAGTGGTGGCAGCAG14770.14559168678368545No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14500.14293022737734862No Hit
GCATGGGATGACCGATGGGGTGGTCGACTGTTCGGCGGAGGGACCACGCT14390.14184592910069285No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA14210.14007162282980162No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14170.13967733254738138No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT13740.13543871201136343No Hit
ACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACAGTCGACCACCCCATC13580.13386155088168236No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13240.13051008348111004No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG13030.12844005949840362No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG12870.12686289836872253No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12810.12627146294509212No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12680.12499001952722623No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12640.12459572924480597No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12560.12380714867996542No Hit
CTATTATTGTGCGAGAGACTGGGTATCAGAACATGCCCCCTACGGAATGG12490.12311714068572994No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12360.12183569726786406No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12220.12045568127939311No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT12220.12045568127939311No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12130.1195685281439475No Hit
ACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTCTATTATTGT12080.11907566529092216No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12010.11838565729668667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11640.11473847218429917No Hit
GTCCTGGAAGTTCTCTGCATATGTTGTTGTGGTTTCCAAAGGGAAGATCC11620.11454132704308902No Hit
GTCCAGGGGCCTGTCGCACCCAGCTAATAGCAGCTTTACTGAAGGTGCCT11610.11444275447248396No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT11450.11286559334280288No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG11440.1127670207721978No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC11360.11197844020735725No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11330.11168272249554205No Hit
GTATACTGGTACCAACAATTCCCAGGAACGGCCCCCAAACTCCTCATCTA11320.111584149924937No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT11290.11128843221312179No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11280.11118985964251671No Hit
CCCCATCGGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGA11080.10921840823041534No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11060.10902126308920523No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTAGTGGTGGCAGCAGCTACAGG10950.10793696481254947No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10780.10626123111226332No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10780.10626123111226332No Hit
GTCTACGAGCACAGCCCACATGGAACTGAGCAGCCTGAGATCTGAGGACA10760.10606408597105318No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10750.10596551340044812No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10740.10586694082984303No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10670.10517693283560757No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10660.1050783602650025No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT10610.10458549741197715No Hit
CAATAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTG10540.1038954894177417No Hit
TTCCAGGACAGACTCACGATTACCGCGGACGAGTCTACGAGCACAGCCCA10480.10330405399411127No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10430.10281119114108593No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10410.10261404599987581No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT10400.10251547342927073No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10260.10113545744079978No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA10200.10054402201716936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17050.083.361091
TATCAAC19900.070.3035352
ATCAACG19800.068.837513
TCAACGC19850.068.664114
CAACGCA19950.068.319935
AACGCAG21150.064.3387766
CGCAGAG25750.052.829628
CAGCGCT5850.051.6756949
ACGCAGA28850.047.1529547
GCAGAGT28900.047.0713739
GCAGCGC7000.045.24268
GGCAGCG7700.040.194877
CAGGGCA9700.035.6860124
GAATACG1051.9343165E-434.2746969
GTGCTAG2353.6379788E-1133.7711941
GTACATG23350.032.134241
ACATGGG24050.031.1850723
TACATGG25150.029.5343722
TATAGAG2003.409441E-728.8461935
CCTATAT2502.590241E-928.8461913
Produced by FastQC (version 0.11.5)