FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1014481
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACCGATGGGGTGGTCGACTGTTCGG31630.31178504082383013No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC31620.31168646825322505No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT28170.2776789313944766No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27760.2736374559996688No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26540.2616116023858505No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA24340.23992563685273555No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG22670.22346401756168915No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACAGTCGACCACCCCAT21880.2156767844838888No Hit
ATAATAGACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTGGG21360.21055101081242528No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20920.20621381770580227No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT20580.20286235030522998No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20260.1997080280458678No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20230.19941231033405257No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20190.1990180200516323No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC19730.19448368180379919No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19420.19142793211504208No Hit
GTCTATTATTGTGCGAGAGACTGGGTATCAGAACATGCCCCCTACGGAAT18820.185513577878738No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17740.17486774025339066No Hit
CCCCTGGACAGGGGCTTGTGTGGATGGGAGGGATCTTCCCTTTGGAAACC17430.17181199056463353No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC16940.1669819346049852No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16930.16688336203438014No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACAGTCG16750.16510905576348892No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG16550.16313760435138755No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG16330.16096900779807605No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16120.15889898381536963No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15890.15663181469145307No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14770.14559168678368545No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14670.14460596107763476No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14490.14283165480674354No Hit
TATGTATACTGGTACCAACAATTCCCAGGAACGGCCCCCAAACTCCTCAT14450.1424373645243233No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14420.14214164681250807No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14330.14125449367706244No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14050.13849446170012056No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13780.13583300229378373No Hit
GCATGGGATGACCGATGGGGTGGTCGACTGTTCGGCGGAGGGACCACGCT13530.133368688028657No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13470.13277725260502662No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTAGTGGTGGCAGCAG13450.13258010746381646No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA13430.13238296232260632No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13150.1296229303456644No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13100.1291300674926391No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA13000.12814434178658843No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12970.1278486240747732No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12770.12587717266267184No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT12550.12370857610936034No Hit
GTCCTGGAAGTTCTCTGCATATGTTGTTGTGGTTTCCAAAGGGAAGATCC12290.12114568927362858No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12010.11838565729668667No Hit
GTCCAGGGGCCTGTCGCACCCAGCTAATAGCAGCTTTACTGAAGGTGCCT11930.11759707673184613No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11850.11680849616700559No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11700.11532990760792956No Hit
GTATACTGGTACCAACAATTCCCAGGAACGGCCCCCAAACTCCTCATCTA11550.11385131904885357No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT11460.11296416591340794No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG11370.11207701277796234No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11200.11040127907767618No Hit
CTATTATTGTGCGAGAGACTGGGTATCAGAACATGCCCCCTACGGAATGG11170.11010556136586097No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11090.10931698080102042No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT11090.10931698080102042No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10990.10833125509496974No Hit
ACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAACAGTCGACCACCCCATC10960.10803553738315455No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG10880.107246956818314No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT10760.10606408597105318No Hit
ACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTCTATTATTGT10480.10330405399411127No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10430.10281119114108593No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10320.10172689286443018No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10310.10162832029382513No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10300.10152974772322004No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10290.10143117515261499No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10270.10123403001140484No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10250.1010368848701947No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10190.1004454494465643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.068.161651
ATCAACG12150.062.7480933
TCAACGC12200.062.490934
CAACGCA12750.060.353385
TATCAAC12800.059.57052
AACGCAG13850.056.0487566
CGCAGAG16200.047.5543988
GCAGAGT18500.041.6422279
GTGCTAG2550.039.501091
GAATACG1701.9590516E-938.116729
GTACATG24900.037.5634271
CCTATAC1355.2923133E-737.293683
ACATGGG24850.036.468263
ACGCAGA21250.036.2532357
TACATGG25750.035.478222
TATTGAG13150.033.9073565
CAGCGCT5750.031.3035839
GGTTTAT9900.031.250321
TTATTGA14150.031.00594
GGCAGCG6450.030.13887
Produced by FastQC (version 0.11.5)