FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933512
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGAGTGATCATCGGCCCACAGGGGTCCCTGACCGGTTCTCTGGCT34250.3668940517101012No Hit
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG25390.2719836488443641No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21630.23170564491940115No Hit
CTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA20390.21842247341223253No Hit
GTATATTACTGTGCGAGGATGGACCACGGGGAAACTTCAATCCCGAACTG19970.21392333467593347No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGG19570.20963844064136294No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT18980.2033182219403714No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17320.18553591169690373No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC16570.177501735382084No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA14960.16025503689293763No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC14880.15939805808602353No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14080.15082827001688248No Hit
CTCTTATCCCATGCAGCACAGAAATAATCAGACTCATCCTCGGACCGGAG13970.1496499241573756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.14922143475391852No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13930.14922143475391852No Hit
GGATAAGAGCCTGAGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCG13840.14825733359614018No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA13840.14825733359614018No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13690.14665049833317623No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG13680.14654337598231196No Hit
CTCTAATAGTGGTGACACAGGCTACGCACAGAAGTTCCAGGGCAGAGTCA13450.1440795619124339No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT13150.14086589138650601No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA13000.13925905612354209No Hit
GCCGTATATTACTGTGCGAGGATGGACCACGGGGAAACTTCAATCCCGAA12640.13540265149242858No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT12610.1350812844398358No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12530.1342243056329217No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12320.13197473626477219No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAGT12200.130689268054401No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGC12050.12908243279143708No Hit
ATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGGCTG11540.12361919289735963No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11410.12222660233612423No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11340.12147674588007437No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11170.1196556659153819No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC11110.11901293181019634No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11100.11890580945933207No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10930.11708472949463959No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10810.11579926128426844No Hit
CTCCCAGGTGCACCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG10800.11569213893340417No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT10780.11547789423167565No Hit
CATTATGTACACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCT10710.1147280377756258No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10710.1147280377756258No Hit
GCCTACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTA10490.112371346056612No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10330.1106573884427838No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10310.11044314374105528No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10290.11022889903932676No Hit
ACATGGAACTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTACTGT10150.10872918612722707No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG10020.10733659556599166No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9870.10572976030302769No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGATGGATGAACTCTAATAGTGGT9830.10530127089957067No Hit
ATATTACTGTGCGAGGATGGACCACGGGGAAACTTCAATCCCGAACTGGT9700.10390868033833522No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT9600.10283745682969261No Hit
TCTATAGGAGTGATCATCGGCCCACAGGGGTCCCTGACCGGTTCTCTGGC9440.10112349921586439No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9350.10015939805808603No Hit
CTATTAGAGTTCATCCATCCCATCCACTCAAGCCCCTGTCCAGGGGCCTG9340.10005227570722176No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9340.10005227570722176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)