FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933512
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGGAGTGATCATCGGCCCACAGGGGTCCCTGACCGGTTCTCTGGCT33490.3587527530444172No Hit
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGG24730.2649135736873227No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21190.22699226148137358No Hit
CTCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA20600.22067204278038202No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCT19940.2136019676233407No Hit
GTATATTACTGTGCGAGGATGGACCACGGGGAAACTTCAATCCCGAACTG19910.21328060057074788No Hit
GTCCTGGGCCCAGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCC17590.18842821517023883No Hit
GTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC17320.18553591169690373No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGG17060.1827507305744329No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTACTGACTCAGCCAC14610.15650575461268842No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14540.15575589815663857No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14190.1520066158763894No Hit
GGATAAGAGCCTGAGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCG13780.1476145994909546No Hit
AGTCTGTACTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG12870.13786646556230664No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12870.13786646556230664No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12770.13679524205366403No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA12690.1359382632467499No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA12580.13475991738724302No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA12350.13229610331736497No Hit
CTCTAATAGTGGTGACACAGGCTACGCACAGAAGTTCCAGGGCAGAGTCA12110.12972516689662264No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12000.12854682103711573No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT11750.12586876226550917No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAGT11550.1237263152482239No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT11470.12286933644130982No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11460.12276221409044555No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10980.11762034124896091No Hit
GCCGTATATTACTGTGCGAGGATGGACCACGGGGAAACTTCAATCCCGAA10740.1150494048282186No Hit
CATTATGTACACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCT10620.11376393661784745No Hit
ATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGTTCCATGTAGGCTG10620.11376393661784745No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10610.11365681426698318No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC10550.11301408016179759No Hit
CTCCCAGGTGCACCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG9770.10465853679438508No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGC9760.10455141444352081No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9590.10273033447882834No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9540.10219472272450703No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9500.10176623332104996No Hit
CTCTTATCCCATGCAGCACAGAAATAATCAGACTCATCCTCGGACCGGAG9450.10123062156672864No Hit
CTATTAGAGTTCATCCATCCCATCCACTCAAGCCCCTGTCCAGGGGCCTG9410.10080213216327161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5350.060.5606351
GTATCAA12200.060.1966131
TCAACGC13750.052.307354
ATCAACG13900.051.222713
TATCAAC14450.049.770762
CAACGCA14450.049.280995
AACGCAG15150.047.003986
GAATACG2450.046.9749079
ACGCAGA16950.041.1636667
GTGCTAG3550.040.5633051
CGCAGAG18150.038.8384138
GTACACG7100.038.497925
TACACGG8200.034.2107626
ACACGGG8750.031.2383127
ATACGGC13650.031.0907636
TATACGG13650.031.0907635
GTACATG24800.029.9031721
TACGGCC14200.029.8865437
ACGGGGT9150.029.8727059
AATATAC15350.029.5171433
Produced by FastQC (version 0.11.5)