FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919343
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTACACCGTCCCAAAGATCTCGCACAGTAATACACGGCCGTGTCGTC42610.4634831613445689No Hit
GTGTAATGGTGATAGATGTTCTTCGTACTACTATTATGTTTTGGACGTCT36870.40104726962624393No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCCCCTCAGTTCCATGTAGG27040.2941230857253495No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18220.1981850082069478No Hit
GTGTATTACTGTGCGAGATCTTTGGGACGGTGTAATGGTGATAGATGTTC17610.19154983504524428No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17100.18600239518873804No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16630.18089004865431074No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16550.1800198620101529No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15520.16881620896662072No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14230.15478444932957558No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13920.15141247608346395No Hit
CCCCTGGAGAGGGGCCTGAGTGGATGGGCTGGATGAACGCTTACAATGGC13790.14999842278670747No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13530.14717031619319448No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13070.14216674298928691No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12410.13498770317498476No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCACAGTGTTGCTG12340.13422628986134663No Hit
ACTATGCACAGACCCTCCAGGGCAGAGTCTCCATGACCACAGACACATCC11830.1286788500048404No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG10650.1158435970035123No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT10630.11562605034247282No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10570.11497341035935445No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10290.11192775710480202No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC10250.1114926637827231No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT10150.1104049304775258No Hit
CCTTTATTAATTATGGCATCAGCTGGGTACGACAGGCCCCTGGAGAGGGG10130.11018738381648634No Hit
GGGTACGACAGGCCCCTGGAGAGGGGCCTGAGTGGATGGGCTGGATGAAC9770.10627154391777607No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9570.10409607730738146No Hit
CTCCAGGGCAGAGTCTCCATGACCACAGACACATCCACAAACACAGCCTA9530.10366098398530255No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9410.1023557040190658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.10181183736646714No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9300.10115919738334876No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCAC9200.10007146407815147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17350.054.8018841
ATCAACG17350.053.9480673
TCAACGC17400.053.790124
CAACGCA17900.051.8853875
AACGCAG19150.048.498616
TATCAAC19300.048.4973562
CGCAGAG20900.044.437728
GGTATCA7900.043.765831
GAATACG2800.043.711869
TACACCG16450.040.2652445
CCGTCCC17250.039.6499759
GTGCTAG4050.037.3495451
CCATTAC18100.037.010541
ATTACAC20150.036.803933
GTACACG8700.034.756695
ACGCAGA27600.033.6503037
CTAGAGA4750.033.345524
CTATACA2456.002665E-1132.3247384
CATTACA21050.031.8099382
ACACGGG9650.030.5889767
Produced by FastQC (version 0.11.5)