FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919343
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTACACCGTCCCAAAGATCTCGCACAGTAATACACGGCCGTGTCGTC42870.46631126793808186No Hit
GTGTAATGGTGATAGATGTTCTTCGTACTACTATTATGTTTTGGACGTCT36470.39669633640545476No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCCCCTCAGTTCCATGTAGG28810.3133759652273417No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20940.22777135410831428No Hit
GTGTATTACTGTGCGAGATCTTTGGGACGGTGTAATGGTGATAGATGTTC17160.18665503517185641No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16410.1784970353828767No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15860.17251450220429154No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15840.17229695554325208No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15670.17044780892441666No Hit
CCCCTGGAGAGGGGCCTGAGTGGATGGGCTGGATGAACGCTTACAATGGC13710.14912823614254964No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13450.14630012954903665No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13240.14401588960812234No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12420.13509647650550446No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCACAGTGTTGCTG12150.13215959658147178No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11670.12693847671652472No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC11610.12628583673340635No Hit
ACTATGCACAGACCCTCCAGGGCAGAGTCTCCATGACCACAGACACATCC11520.12530687675872879No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11290.122805090156775No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10900.11856293026650555No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10680.11616991699507147No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10670.11606114366455175No Hit
GGGTACGACAGGCCCCTGGAGAGGGGCCTGAGTGGATGGGCTGGATGAAC10480.1139944503846769No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10390.11301549040999932No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT10270.11171021044376254No Hit
CCTTTATTAATTATGGCATCAGCTGGGTACGACAGGCCCCTGGAGAGGGG10070.10953474383336796No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9920.10790314387557201No Hit
CTCCAGGGCAGAGTCTCCATGACCACAGACACATCCACAAACACAGCCTA9840.10703295723141416No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCAC9550.10387853064634202No Hit
CCTCATATACAAGCAGCAACACTGTGATATTCGGCGGAGGGACCAAGGTG9350.10170306403594742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)