FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066711
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTTAGGCTCCTCAGTTCCATGTAGG42560.3989834172517205No Hit
GTGTATTACTGTGCGAGAGTGCATTTTTATGGTGCAGGGAGTATTAGCGA27150.2545206714845914No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24390.22864674686958325No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23700.22217826571583119No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22580.21167870210394382No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA20660.19367945019785116No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20050.18796093787351964No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19760.1852423008668702No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19180.1798050268535714No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19130.17933629633518358No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17940.1681805099975532No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17510.16414942753941789No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16560.15524354769004914No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15550.14577519121861499No Hit
CTCCCAGGTTCAGCTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGG14300.13405692825891924No Hit
GTATTACTGTGCGAGAGTGCATTTTTATGGTGCAGGGAGTATTAGCGAAA13520.1267447321720691No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13420.12580727113529344No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG13400.1256197789279383No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13270.12440107958012994No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12950.12140120426244784No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGCTGGATCAGCAATGACGGCAAA12940.12130745815877027No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12790.11990126660360678No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12720.11924504387786383No Hit
GTCCAAGGTGGCGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12700.11905755167050869No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT12690.11896380556683113No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12390.11615142245650414No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12300.11530770752340605No Hit
GTTTGTGTGTTTGCCGTCATTGCTGATCCAGCCCATCCACTCAAGCCCTT12210.11446399259030797No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12170.11408900817559771No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12080.11324529324249961No Hit
CAGTAATACACGGCCGTGTCGTCAGATCTTAGGCTCCTCAGTTCCATGTA11860.11118287896159315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11810.11071414844320533No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11660.10930795688804183No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCC11610.10883922636965401No Hit
CACACAAACTATGCACAGAAGTTCCAGGGCAGAGTCACCATGACCTCAGA11580.1085579880586213No Hit
GTGCAGGGAGTATTAGCGAAAACTGGTCCGACCCCTGGGGCCAGGGAACC11540.10818300364391104No Hit
GTCCAGGGGCCTGTCGCACCCAGGTGAGACCAAAGTTGGTAAAGGTGTAA11510.10790176533287835No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11490.10771427312552323No Hit
ATACACGGCCGTGTCGTCAGATCTTAGGCTCCTCAGTTCCATGTAGGCTG11450.10733928871081297No Hit
GCCTACATGGAACTGAGGAGCCTAAGATCTGACGACACGGCCGTGTATTA11330.10621433546668217No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.10537062053358408No Hit
ACCTTGGACCCTCCGCCGAAAGTGAGGGGGGTAGTGAAAGTCTGTTGACA11240.10537062053358408No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11110.10415192118577571No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG11090.10396442897842059No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCACTG11030.10340195235635519No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11020.10330820625267761No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10940.10255823742325709No Hit
ACATGGAACTGAGGAGCCTAAGATCTGACGACACGGCCGTGTATTACTGT10890.10208950690486927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19500.065.025851
TCAACGC19700.063.2270244
ATCAACG20200.062.021343
TATCAAC21000.059.658622
CAACGCA21100.059.0318685
AACGCAG22850.054.510836
CGCAGAG24950.050.4975178
CAGCGCT5550.044.1050649
ACGCAGA32500.038.7665567
GCAGCGC7000.034.9690138
ATACACG22750.034.8124164
GTAATAC24350.032.250431
AATACAC25500.031.341953
GTACATG24100.030.193421
GAATACG2651.5097612E-1029.884739
ACATGGG23000.029.4268173
GGCAGCG8100.029.331317
TAATACA27450.028.590872
GTATATA4850.028.2240831
GTGTAGA4400.027.8358351
Produced by FastQC (version 0.11.5)