FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596810
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA23290.3902414503778422No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19830.3322665504934569No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16530.2769725708349391No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14320.23994236021514384No Hit
GTGTATTACTGTGCGAGAGCGGGGTATAGCAGTGGCTGGACGGTAGGGTA13120.21983545852113737No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG12660.2121278128717682No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11900.19939344179889748No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11600.19436671637539585No Hit
GCTATACCCCGCTCTCGCACAGTAATACACAGCCGTGTCCTCAGCTCTCA10630.17811363750607395No Hit
CTGCTATACCCCGCTCTCGCACAGTAATACACAGCCGTGTCCTCAGCTCT9680.1621956736649855No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC9630.1613578860944019No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9550.16001742598146815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.1521422228179823No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8980.15046664767681509No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8760.14678038236624721No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA8370.14024563931569511No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8000.13404601129337645No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT7580.1270085957004742No Hit
CCGTAGTAGTAGTACCCTACCGTCCAGCCACTGCTATACCCCGCTCTCGC7140.11963606507933847No Hit
GTATTTATTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT7130.11946850756522176No Hit
GTATAGCAGTGGCTGGACGGTAGGGTACTACTACTACGGTATGGACGTCT6830.11444178214172014No Hit
GGGTATAGCAGTGGCTGGACGGTAGGGTACTACTACTACGGTATGGACGT6530.10941505671821852No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG6510.1090799416899851No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6510.1090799416899851No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT6330.1060639064358841No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTAATAAATACTA6160.10321542869589986No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC6130.1027127561535497No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGG6130.1027127561535497No Hit
GTACCCTACCGTCCAGCCACTGCTATACCCCGCTCTCGCACAGTAATACA6110.10237764112531628No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTAC6040.10120473852649924No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6000.10053450847003233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)