FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596810
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA22370.3748261590791039No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG20820.3488547443910122No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15440.2587088017962166No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT13590.22771066168462326No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG13180.2208408036058377No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG12270.20559306982121614No Hit
GTGTATTACTGTGCGAGAGCGGGGTATAGCAGTGGCTGGACGGTAGGGTA11990.20090145942594798No Hit
GCTATACCCCGCTCTCGCACAGTAATACACAGCCGTGTCCTCAGCTCTCA10230.17141133694140515No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC10150.17007087682847136No Hit
CTGCTATACCCCGCTCTCGCACAGTAATACACAGCCGTGTCCTCAGCTCT9990.16738995660260386No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT9790.16403880632026943No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC9660.16186055863675206No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG8910.149293745077998No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT8810.1476181699368308No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA8630.14460213468272984No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8220.1377322766039443No Hit
GTATTTATTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT7350.12315477287578962No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT6960.1166200298252375No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT6870.11511201219818702No Hit
CCGTAGTAGTAGTACCCTACCGTCCAGCCACTGCTATACCCCGCTCTCGC6870.11511201219818702No Hit
GTATAGCAGTGGCTGGACGGTAGGGTACTACTACTACGGTATGGACGTCT6760.1132688795429031No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC6670.1117608619158526No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGG6110.10237764112531628No Hit
GGGTATAGCAGTGGCTGGACGGTAGGGTACTACTACTACGGTATGGACGT6000.10053450847003233No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)