FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences790758
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACACAGCCGTGTCATCGGTCCTCAGACTGTTCATTTGCAGATACA20180.2551981769390888No Hit
GTATTATTGTGCGCGAGAACTGCGATTTGGTTCGGGGTGGTGGGCTCCGG15430.19512923043459568No Hit
CAATAATACACAGCCGTGTCATCGGTCCTCAGACTGTTCATTTGCAGATA14940.18893264437413215No Hit
GTGTATTATTGTGCGCGAGAACTGCGATTTGGTTCGGGGTGGTGGGCTCC14190.1794480738734227No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14080.17805700353331866No Hit
GTCTGAGGACCGATGACACGGCTGTGTATTATTGTGCGCGAGAACTGCGA13960.17653947225320515No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13940.17628655037318625No Hit
GTCCTCAGACTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTCGTCTCT13150.16629613611243893No Hit
GTGTGAGGTGCAGTTGGTGGAGTCTGGGGGAGGCCCGGTCAAGCCGGGGG12360.15630572185169167No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12220.15453526869155923No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11020.1393599558904241No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA10660.1348073620500836No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10500.13278398700993224No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9950.125828635309412No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG9380.1186203617288728No Hit
GTCTGGGGGAGGCCCGGTCAAGCCGGGGGGGTCCCTGAGACTCTCTTGTG9210.116470525748712No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9110.1152059163486174No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8870.11217085378839038No Hit
GTCCATACCTTGCATGCAGTAATAAACGCCAACATCCTCAGCCTCCACCC8740.11052686156826741No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8570.1083770255881066No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8470.10711241618801201No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8400.10622718960794579No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA8260.10445673644781336No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8080.10218043952764311No Hit
CTCTTGTGTAGCCTCTGGATTCACCTTCAGTAACTATCGCATGAACTGGG8010.10129521294757687No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7910.10003060354748228No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8800.058.9290431
TCAACGC8550.058.1249434
ATCAACG9250.054.504943
TATCAAC9900.050.926332
CAACGCA9750.050.232395
AACGCAG10450.048.2215966
GGTATCA3750.044.1749571
CGCAGAG12500.040.889168
GTACATG20100.040.849651
TACATGG20100.040.491322
AATCCGC951.07253094E-437.8883976
ACATGGG20850.037.6530383
GCAGAGT15000.034.554229
CATGGGG14100.030.6486764
CAGCCGT10100.029.2228369
ATAATAC11600.028.5613961
CATGGGA10100.028.5245114
CAGAGTA14400.027.9953149
TCCGCTA1050.00797870827.4239838
TAGGACT2757.2523108E-926.1906874
Produced by FastQC (version 0.11.5)