FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862382
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAACTTACCCGTTCACTTTCGGCCCTGGGACCACAGTGGATATCA31840.3692099324893145No Hit
GTTGTAAGGCTGTTCATTTGGAGATACAGTGAGTTCTTTGAATCATCTCT26340.3054330911359467No Hit
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT23770.27563191253991853No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21530.24965734442509235No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18700.2168412606014504No Hit
ATCTACCACAGTAATAAACGGCCGTGTCCTCGGTTGTAAGGCTGTTCATT17210.19956353448935624No Hit
GTAATAAACGGCCGTGTCCTCGGTTGTAAGGCTGTTCATTTGGAGATACA17170.19909970291587717No Hit
CCTTTAGACCATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATC16550.1919103135269521No Hit
GTCTAAAGGAGATAACCCGAGGGGCGATCTACCACAGTAATAAACGGCCG16260.18854753461922907No Hit
GCCTTACAACCGAGGACACGGCCGTTTATTACTGTGGTAGATCGCCCCTC16230.18819966093911977No Hit
GTGGTAGATCGCCCCTCGGGTTATCTCCTTTAGACCATTGGGGCCAGGGA14360.16651553487897475No Hit
CAACATGGACTGGGCCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG13610.15781869287624278No Hit
CTTACAAGGTGTCCAGTGTGAGGTGCAGCTCGTGGAGTCTGGGGGAGGCT13340.15468782975525927No Hit
GATCTATGCTGCATCCAGTTTACTAAGTGGGGTCCCATCAAAGTTCAGCG13010.1508612192740572No Hit
CTCCTGTGTAGCCTCAGGATTCACCTTCAGTGACCACAACATGGACTGGG12970.15039738770057817No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12320.1428601246315438No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12240.14193246148458571No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11910.13810585100338366No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11700.1356707352426187No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11630.13485902998903038No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11570.1341632826288118No Hit
GTACATGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGT11500.1333515773752235No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11020.12778559849347507No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10830.1255823985194496No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10540.12221961961172659No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10300.11943663017085238No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10270.11908875649074308No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10230.11862492491726405No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTGGCAA10030.11630576704986886No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9980.11572597758302006No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.1156100196896503No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9970.1156100196896503No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9770.1132908618222551No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9510.11027595659464134No Hit
GTGTGAGGTGCAGCTCGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGCG9280.10760892504713689No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9160.10621743032669977No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9140.10598551453996025No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8870.10285465141897673No Hit
GTTTATTACTGTGGTAGATCGCCCCTCGGGTTATCTCCTTTAGACCATTG8790.10192698827201865No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA8710.10099932512506059No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17500.062.617151
TCAACGC18050.059.504034
ATCAACG18400.058.7639163
GGTATCA7850.057.8573571
CAACGCA19100.055.855465
TATCAAC19800.055.337012
AACGCAG20550.051.914326
ACGCAGA22600.047.4939087
CGCAGAG22800.047.0527278
GCAGAGT26550.040.675699
GAATACG2350.039.8275579
TACGCTA951.0719093E-437.8926129
GTGCTAG3600.034.0434951
CGCTATA900.003731699732.0372162
GTACATG22350.031.6108591
TACATGG22500.031.3964752
TATACGC1204.2125123E-430.0157247
GTGTAGA4000.028.8368431
ACTTACC18300.027.5410378
CTTACCC18400.026.998499
Produced by FastQC (version 0.11.5)