FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862382
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAACTTACCCGTTCACTTTCGGCCCTGGGACCACAGTGGATATCA29900.3467141011755811No Hit
GTTGTAAGGCTGTTCATTTGGAGATACAGTGAGTTCTTTGAATCATCTCT24060.27899469144764155No Hit
GTTTTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT23110.26797869157751436No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20480.23748176562126763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17110.19840395555565862No Hit
ATCTACCACAGTAATAAACGGCCGTGTCCTCGGTTGTAAGGCTGTTCATT16880.19573692400815415No Hit
GTAATAAACGGCCGTGTCCTCGGTTGTAAGGCTGTTCATTTGGAGATACA16220.18808370304575003No Hit
GTCTAAAGGAGATAACCCGAGGGGCGATCTACCACAGTAATAAACGGCCG15580.18066239787008542No Hit
GCCTTACAACCGAGGACACGGCCGTTTATTACTGTGGTAGATCGCCCCTC15080.17486450320159744No Hit
CCTTTAGACCATTGGGGCCAGGGAACCCAGGTCACCGTCTCCTCAGCATC14910.17289321901431154No Hit
CTTACAAGGTGTCCAGTGTGAGGTGCAGCTCGTGGAGTCTGGGGGAGGCT13270.15387612450167093No Hit
GTGGTAGATCGCCCCTCGGGTTATCTCCTTTAGACCATTGGGGCCAGGGA12660.14680269300611562No Hit
CTCCTGTGTAGCCTCAGGATTCACCTTCAGTGACCACAACATGGACTGGG12390.1436718298851321No Hit
CAACATGGACTGGGCCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTG12350.14320799831165307No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12270.142280335164695No Hit
GATCTATGCTGCATCCAGTTTACTAAGTGGGGTCCCATCAAAGTTCAGCG12130.14065692465751836No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA11810.13694627206968607No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11610.13462711420229087No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11370.13184412476141663No Hit
GTACATGGGAGCTCTGAGAGCGGAGCCCCAGCCCCAGAATTCCCAGGTGT10970.12720580902662626No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10630.12326324065205442No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10520.12198770382498708No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10430.12094408278465922No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10420.12082812489128947No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10130.11746534598356645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10100.11711747230345718No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10020.1161898091564991No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTGGCAA9800.11363873550236438No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9640.11178340920844823No Hit
GTGTGAGGTGCAGCTCGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGCG9150.10610147243333No Hit
GTTTATTACTGTGGTAGATCGCCCCTCGGGTTATCTCCTTTAGACCATTG9120.10575359875322074No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9020.10459401981952313No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA8970.10401423035267435No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8950.10378231456593481No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG8750.10146315669853964No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8740.10134719880516986No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG353.527902E-582.4221951
CGCATAC450.009897822548.0098159
GGTATCA5400.044.072981
TCAACGC10700.043.800684
ATCAACG10750.043.5969543
GTATCAA11600.042.898621
CAACGCA11700.040.05475
AACGCAG12100.038.6900866
TATCAAC12800.037.7412342
GTTAGAC800.002092134436.0513273
GAATACG3300.034.916239
GTGCTAG4150.034.7563481
ACGCAGA14050.033.318357
CGCAGAG14500.032.2843368
GTACATG20950.028.5723741
TACATGG21250.028.1624492
GCAGAGT17600.027.4146969
TCTATAC2405.558286E-827.0384943
TCTACAC7250.026.852023
TGCTAGA5350.025.606552
Produced by FastQC (version 0.11.5)