FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856288
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCAGGTGCAGAGACA30740.3589913673904107No Hit
GTATATTATTGTGCAAGAGTCCAACAGAGCGACCGAGTGGGAGCTCCTTT24670.2881040023917187No Hit
GTATTACTGTCAGCAGTATGGTGGCTCAGCCTGGACGTTCGGCCAAGGGA16610.19397679285474045No Hit
ATATACAGCCGTGTCCTCGTCTCTCAGGCTGTTCAGGTGCAGAGACAGTG16430.1918746963638402No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14760.17237191225382115No Hit
GCTGTATATTATTGTGCAAGAGTCCAACAGAGCGACCGAGTGGGAGCTCC13520.15789080309428605No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC11430.13348312717216637No Hit
ATATATGGTTCCACTACTTCTACTAATATATGAAACCCACTCCAGCCCCT11240.13126424754288277No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGACTGCTGATGGTGAGAAT11230.13114746440449943No Hit
GCGTAATATATGGTTCCACTACTTCTACTAATATATGAAACCCACTCCAG11130.12997963302066595No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10750.12554187376209874No Hit
ATATTACGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGACAATG10010.11689992152173101No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.11433069247729737No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9570.1117614634328637No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9470.11059363204903024No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9090.10615587279046303No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT8970.10475447512986284No Hit
ACCATATATTACGCAGACTCTGTGAAGGGCCGATTCACCGTCTCCAGAGA8770.1024188123621959No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)