FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446239
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCG22240.4983876353254646No Hit
CCCATATATAGCACACAGTGATAATAAGATTCATCATCTGCCTGGGCCCC15260.341969213806951No Hit
ATATAGCACACAGTGATAATAAGATTCATCATCTGCCTGGGCCCCCGTGA13700.3070103688830425No Hit
GTGCTATATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCT11280.25277934021903065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.20280611959062295No Hit
TATTATCACTGTGTGCTATATATGGGTAGTGGCATTTGGGTGTTCGGCGG8770.1965314551171009No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8660.19406640835964584No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG8500.19048088580334752No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8120.18196526973213906No Hit
GTGATAATAAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG7510.16829546498625178No Hit
GTGGTGACCCAGGAGCCATCGTTTTCAGTGTCCCCTGGAGGGACAGTCAC7480.16762317950694583No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7110.159331658595506No Hit
CTCATCTACAGCACAAATATTCGCTCTTCTGGGGTCCCTGATCGCTTCTC7080.15865937311620007No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6910.15484975540013313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6750.1512642328438348No Hit
CTACTAGTTACTGCCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCA6600.14790280544730514No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6540.1465582344886933No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA6520.146110044169156No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTA6510.14588594900938737No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6400.14342090225193227No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA6130.13737033293817888No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6130.13737033293817888No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC5910.1324402394232687No Hit
GTCTCTACTAGTTACTGCCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC5890.1319920491037314No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT5850.1310956684646568No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG5710.12795833622789582No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5570.12482100399113479No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG5480.12280414755321699No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG5430.12168367175437378No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT5410.12123548143483649No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA5340.11966681531645597No Hit
GTAATAAAGGGCCGAGTCCGCGGCGGTCACAGAGTTCAGCCACAGGGAGA5320.11921862499691868No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTTTCAGTGTCCCCTGGAG5300.11877043467738141No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG5240.11742586371876955No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT5110.11451262664177718No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG5110.11451262664177718No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG5080.11384034116247123No Hit
CCCCTGGAGGGACAGTCACATTCACTTGTGGCTTGAGCTCTGGCTCAGTC5040.11294396052339666No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT4920.11025481860617292No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG4790.10734158152918055No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG4740.10622110573033734No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT4640.1039801541326509No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC4620.10353196381311361No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT4590.10285967833380766No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCT4530.1015151073751958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)