FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446239
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCG18460.4136796649329171No Hit
CCCATATATAGCACACAGTGATAATAAGATTCATCATCTGCCTGGGCCCC14630.3278512187415264No Hit
ATATAGCACACAGTGATAATAAGATTCATCATCTGCCTGGGCCCCCGTGA11560.25905400469255263No Hit
GTGCTATATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCT10900.24426372414782213No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9040.20258202443085432No Hit
TATTATCACTGTGTGCTATATATGGGTAGTGGCATTTGGGTGTTCGGCGG7810.1750183197793111No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7330.16426175211041616No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7150.16022803923458057No Hit
GTGGTGACCCAGGAGCCATCGTTTTCAGTGTCCCCTGGAGGGACAGTCAC7090.1588834682759687No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6750.1512642328438348No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG6710.15036785220476023No Hit
GTGATAATAAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG6650.14902328124614836No Hit
CTCATCTACAGCACAAATATTCGCTCTTCTGGGGTCCCTGATCGCTTCTC6360.14252452161285767No Hit
GTCTCTACTAGTTACTGCCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC6280.14073176033470852No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA6170.13826671357725345No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT5880.13176795394396276No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA5850.1310956684646568No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT5670.12706195558882125No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG5580.12504509915090342No Hit
CTACTAGTTACTGCCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCA5540.12414871851182886No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA5510.12347643303252294No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTA5280.11832224435784412No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA5180.11608129276015768No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCGTTTTCAGTGTCCCCTGGAG5180.11608129276015768No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT5050.11316805568316529No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA5010.11227167504409073No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT4930.11047891376594157No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC4900.10980662828663564No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG4850.10868615248779241No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT4760.10666929604987463No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG4730.10599701057056869No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC4660.10442834445218818No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG4570.10241148801427037No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT4480.10039463157635259No Hit
GTAATAAAGGGCCGAGTCCGCGGCGGTCACAGAGTTCAGCCACAGGGAGA4470.10017053641658392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTCG250.001064309984.283969
GGTATCA4400.073.42921
GTATCAA9700.062.995741
GTCTAAC400.00685713752.6774751
TCAACGC11450.052.140734
TATCAAC11500.051.3032762
ATCAACG11650.051.245613
CAACGCA11800.049.9989555
AACGCAG12200.048.359656
TAGGACC856.4345426E-541.3156624
GTATACA700.001234290340.135224
ACGCAGA15150.038.943087
AGCAGCG5250.038.58615145
CGCAGAG15300.038.5612878
GACCGCG750.001732466737.4595347
GCAGAGT16450.035.865519
ACTTGCG3352.728484E-1035.57108145
TATTGAG6950.035.3709645
TCTACAC2800.035.1183173
CTAGCAC1001.6752441E-435.1183173
Produced by FastQC (version 0.11.5)