FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630578
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAAAGTCCCGGCACTGATTGGTGAAAGGGACGGTGT11140.17666331524410936No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT6790.10767898658056577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10700.071.3378451
GGTATCA5300.064.743691
TCAACGC12750.058.7693444
CAACGCA13150.056.981685
AACGCAG13250.056.5516326
ATCAACG13850.053.596123
TATCAAC14450.051.3706782
ACGCAGA17000.043.6650737
CGCAGAG16850.043.2225768
GTACATG20900.038.867711
TACATGG21150.038.0771752
ACATGGG22750.036.3226743
GCAGAGT20200.036.0544789
CATGGGG15350.027.3728564
CATGGGA10200.026.7757534
CTAGTAC1050.00912659126.6776753
GTTATAG2001.3929977E-524.5101131
GTATTAG3302.6429916E-923.3429641
GGTATAC1500.001827722823.3429641
GGAGTGT3002.4630936E-823.3429646
Produced by FastQC (version 0.11.5)