FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520893
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTATACAGCTCCACCAACAGAAACTACTTAGCTTGGTACCAGCAAAA23970.46017128277784497No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG21640.4154404071469573No Hit
ATATTATAGTAGTCCTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCA21620.4150564511329582No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19700.37819667378905075No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19050.36571810333408206No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15910.30543700913623334No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG15810.3035172290662382No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15100.28988679056927236No Hit
TTATTACTGTCAGCAATATTATAGTAGTCCTCCGACATTCGGCCAAGGGA15080.28950283455527337No Hit
GTCTATTACTGTACAAGAGGGCAGGGTTCGGATGGCTGGTTCGGCCCCTG14180.2722248139253167No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13800.264929649659335No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC12370.23747679465840393No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12310.23632492661640683No Hit
GTAATAGACAGCCGTGTCCTCGACTGTCAGACTGTCCATTTGCAGATACA11970.2297976743784232No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT11270.21635921388845694No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11230.21559130186045886No Hit
CTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT11180.21463141182546125No Hit
CTCCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA11090.2129036097624656No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT11060.21232767574146705No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC10500.20157690734949404No Hit
CTATAATATTGCTGACAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCT10030.19255394102051668No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9960.19121009497152008No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9800.18813844685952777No Hit
CTTTATTACTGTCAGCAATATTATAGTAGTCCTCCGACATTCGGCCAAGG9740.18698657881753067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.17700372245355572No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9200.17661976643955668No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9180.17623581042555764No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC9090.17450800836256197No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG9040.1735481183275644No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC9000.17278020629956634No Hit
TTATAGTAGTCCTCCGACATTCGGCCAAGGGACCAAGGTGGAAATCAAAC8530.16375723997058897No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8380.16087756986559618No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA7690.14763108738262945No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7620.1462872413336328No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT7270.1395680110886497No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGACTTTGGGCTGAGCTGGG7200.13822416503965307No Hit
GTATAAAATATGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGAC7060.1355364729416598No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT7020.13476856091366174No Hit
GTCCAGCACCCCAACGGCAACAAAGAAAAGAACGTGCCTCTTCCAGTGAT6740.12939317671767522No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6740.12939317671767522No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC6630.12728141864068052No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC6610.12689746262668147No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6290.12075416640269691No Hit
GTACATGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCA6270.12037021038869787No Hit
GTACAAGAGGGCAGGGTTCGGATGGCTGGTTCGGCCCCTGGGGCCAGGGA6180.1186424083257022No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG6180.1186424083257022No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT6170.1184504303187027No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA6010.1153787822067104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5960.11441889217171282No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC5950.11422691416471328No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG5850.11230713409471811No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC5790.111155266052721No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5690.10923548598272582No Hit
GTCTGACAGTCGAGGACACGGCTGTCTATTACTGTACAAGAGGGCAGGGT5680.10904350797572629No Hit
CCCTGACTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC5630.10808361794072871No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC5540.10635581587773305No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5490.10539592584273545No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC5440.10443603580773786No Hit
CAGTAATAAAGTGCCACATCTTCAGCCTGCAGGCTGCGGATGGTGAGAGT5430.10424405780073834No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC5410.10386010178673932No Hit
AACCAGGACAGCCTCCCAAACTGCTCATTTACTGGGCATCTACCCGGGAA5400.10366812377973979No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC5350.10270823374474221No Hit
GTCTGGGACAGATTTCACTCTCACCATCCGCAGCCTGCAGGCTGAAGATG5260.10098043168174654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5350.099.473721
GTATCAA12100.076.968821
TATCAAC15700.058.8739052
ATCAACG16400.055.9340173
TCAACGC16650.055.0941664
CAACGCA16650.054.67365
AACGCAG17300.052.619396
TCACGAT2900.047.898037145
CTTCTAT14800.045.22069145
CGCAGAG20100.044.9409458
ACGCAGA20250.044.6080477
ATAGGTG7800.043.9895974
TAGGTGG8150.042.9596675
TCATAGG8250.041.5901682
GCAGAGT22000.041.377979
GTCATAG8300.041.3396261
GTACATG17300.040.8812181
TACATGG17500.040.814142
GTGGTCA8350.040.2534688
CATGGGG13350.039.3394744
Produced by FastQC (version 0.11.5)